pNubG-Fe65
Catalog No. PVT11259
Packing 2ug
pNubG-Fe65 information
Promoter: ADH1 promoter
Plasmid classification: yeast cell plasmids; yeast hybrid plasmids; two hybrid plasmids
Plasmid size: 8579bp
Prokaryotic resistance: ampicillin Amp
Selection marker: TRP1
Cloning strains: E. coli DH5 and E.
Culture conditions: 37 C, aerobic, LB
Remarks: Positive control pre
pNubG-Fe65 Description
Coexpression of Cub-fused APP and NubG-fused Fe65 from pTSU2-APP and pNubG-Fe65 should lead to robust growth on selective medium as APP and Fe65 interact with each other and consequently initiate the split-ubiquitin mechanism.
