pNubG-Fe65

Catalog No. PVT11259
Packing 2ug
 

pNubG-Fe65 information

Promoter: ADH1 promoter

Plasmid classification: yeast cell plasmids; yeast hybrid plasmids; two hybrid plasmids

Plasmid size: 8579bp

Prokaryotic resistance: ampicillin Amp

Selection marker: TRP1

Cloning strains: E. coli DH5 and E.

Culture conditions: 37 C, aerobic, LB

Remarks: Positive control pre

 

pNubG-Fe65 Description

Coexpression of Cub-fused APP and NubG-fused Fe65 from pTSU2-APP and pNubG-Fe65 should lead to robust growth on selective medium as APP and Fe65 interact with each other and consequently initiate the split-ubiquitin mechanism.