pNZ8048

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  • Model: PVT5204
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pNZ8048

PVT5204    2ug
 

pNZ8048 Plasmid information

Promoter: PnisA
Replicon: repA, repC
Terminator: Terminator
Plasmid classification: secreted expression plasmid of Lactobacillus
Plasmid size: 3349bp
Prokaryotic resistance: chloramphenicol chloramphenicol
Screening marker: chloramphenicol chloramphenicol
Clone strain: DH5 alpha
Culture conditions: 37, aerobic, LB
Expressing host: Lactobacillus
Primers for 5'sequencing: Based on sequence design

pNZ8048 plasmid

pNZ8048 plasmid Sequence

LOCUS       Exported                3349 bp ds-DNA     circular SYN 10-MAY-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pNZ8048
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 10, 2016 from SnapGene Viewer 3.1.2
            
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    6..189
                     /note="PnisA"
     misc_feature    202..252
                     /note="MCS"
     misc_feature    394..446
                     /note="Terminator"
     misc_feature    788..997
                     /note="RepC"
     misc_feature    1266..1964
                     /note="RepA"
     gap             2138..3349
                     /estimated_length="1212"
ORIGIN
        1 agatctagtc ttataactat actgacaata gaaacattaa caaatctaaa acagtcttaa
       61 ttctatcttg agaaagtatt ggtaataata ttattgtcga taacgcgagc ataataaacg
      121 gctctgatta aattctgaag tttgttagat acaatgattt cgttcgaagg aactacaaaa
      181 taaattataa ggaggcactc accatgggta ctgcaggcat gcggtaccac tagttctaga
      241 gagctcaagc tttctttgaa ccaaaattag aaaaccaagg cttgaaacgt tcaattgaaa
      301 tggcaattaa acaaattaca gcacgtgttg ctttgattga tagccaaaaa gcagcagttg
      361 ataaagcaat tactgatatt gctgaaaaat tgtaatttat aaataaaaat caccttttag
      421 aggtggtttt tttatttata aattattcgt ttgatttcgc tttcgataga acaatcaaat
      481 cgtttctgag acgttttagc gtttatttcg tttagttatc ggcataatcg ttaaaacagg
      541 cgttatcgta gcgtaaaagc ccttgagcgt agcgtggctt tgcagcgaag atgttgtctg
      601 ttagattatg aaagccgatg actgaatgaa ataataagcg cagcgtcctt ctatttcggt
      661 tggaggaggc tcaagggagt ttgagggaat gaaattccct catgggtttg attttaaaaa
      721 ttgcttgcaa ttttgccgag cggtagcgct ggaaaatttt tgaaaaaaat ttggaatttg
      781 gaaaaaaatg gggggaaagg aagcgaattt tgcttccgta ctacgacccc ccattaagtg
      841 ccgagtgcca atttttgtgc caaaaacgct ctatcccaac tggctcaagg gtttgagggg
      901 tttttcaatc gccaacgaat cgccaacgtt ttcgccaacg ttttttataa atctatattt
      961 aagtagcttt atttttgttt ttatgattac aaagtgatac actaatttta taaaattatt
     1021 tgattggagt tttttaaatg gtgatttcag aatcgaaaaa aagagttatg atttctctga
     1081 caaaagagca agataaaaaa ttaacagata tggcgaaaca aaaagatttt tcaaaatctg
     1141 cggttgcggc gttagctata gaagaatatg caagaaagga atcagaacaa aaaaaataag
     1201 cgaaagctcg cgtttttaga aggatacgag ttttcgctac ttgtttttga taaggtaatt
     1261 atatcatggc tattaaaaat actaaagcta gaaattttgg atttttatta tatcctgact
     1321 caattcctaa tgattggaaa gaaaaattag agagtttggg cgtatctatg gctgtcagtc
     1381 ctttacacga tatggacgaa aaaaaagata aagatacatg gaatagtagt gatgttatac
     1441 gaaatggaaa gcactataaa aaaccacact atcacgttat atatattgca cgaaatcctg
     1501 taacaataga aagcgttagg aacaagatta agcgaaaatt ggggaatagt tcagttgctc
     1561 atgttgagat acttgattat atcaaaggtt catatgaata tttgactcat gaatcaaagg
     1621 acgctattgc taagaataaa catatatacg acaaaaaaga tattttgaac attaatgatt
     1681 ttgatattga ccgctatata acacttgatg aaagccaaaa aagagaattg aagaatttac
     1741 ttttagatat agtggatgac tataatttgg taaatacaaa agatttaatg gcttttattc
     1801 gccttagggg agcggagttt ggaattttaa atacgaatga tgtaaaagat attgtttcaa
     1861 caaactctag cgcctttaga ttatggtttg agggcaatta tcagtgtgga tatagagcaa
     1921 gttatgcaaa ggttcttgat gctgaaacgg gggaaataaa atgacaaaca aagaaaaaga
     1981 gttatttgct gaaaatgagg aattaaaaaa agaaattaag gacttaaaag agcgtattga
     2041 aagatacaga gaaatggaag ttgaattaag tacaacaata gatttattga gaggagggat
     2101 tattgaataa ataaaagccc ccctgacgaa agtcgacnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnn
//
 
Caution:
Product is for research use only!
 

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