pNZ8148 Plasmid

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  • Model: PVT5205
  • 47 Units in Stock
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pNZ8148  Plasmid

PVT5205  2ug
 

pNZ8148 Plasmid informaiton

Promoter: PnisA
Replicator: repA, repC
Terminator: T
Plasmid classification: expression plasmid of Lactobacilli
Plasmid size: 3167bp
Prokaryotic resistance: chloramphenicol chloramphenicol
Screening markers: chloramphenicol chloramphenicol
Cloned strains: MC1061
Culture conditions: 37 centigrade, aerobic, LB
Expression host: Lactobacilli
5'sequencing primers: Based on sequence design
Use:Lactic Acid Bacteria Plasmids
 

pNZ8148 Description

pNZ8148 contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII. The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. However, this replicon has a broad host range. Plasmids with this replicon can replicate in many Gram-positive bacteria, such as Lactobacillus plantarum and Streptococcus thermophilus. pNZ8148 – In this vector the nisA promoter is followed by an NcoI site for translational fusions at the ATG. It contains a terminator after the MCS. Sequence adaptation for cloning in NcoI can result in a change in the second amino acid of a protein (Mierau and Kleerebezem, 2005).

pNZ8148 vector
 

pNZ8148 Sequence

LOCUS       Exported                3167 bp ds-DNA     circular SYN 29-AUG-2016

FEATURES             Location/Qualifiers

     source          1..3167

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    5..188

                     /note="Pnis"

     misc_feature    393..445

                     /note="Terminator"

     misc_feature    787..996

                     /note="repC"

     misc_feature    1264..1962

                     /note="repA"

     misc_feature    2421..3071

                     /note="Cm"

ORIGIN

        1 agatctagtc ttataactat actgacaata gaaacattaa caaatctaaa acagtcttaa

       61 ttctatcttg agaaagtatt ggtaataata ttattgtcga taacgcgagc ataataaacg

      121 gctctgatta aattctgaag tttgttagat acaatgattt cgttcgaagg aactacaaaa

      181 taaattataa ggaggcactc accatgggta ctgcaggcat gcggtaccac tagttctaga

      241 gagctcaagc tttctttgaa ccaaaattag aaaaccaagg cttgaaacgt tcaattgaaa

      301 tggcaattaa acaaattaca gcacgtgttg ctttgattga tagccaaaaa gcagcagttg

      361 ataaagcaat tactgatatt gctgaaaaat tgtaatttat aaataaaaat caccttttag

      421 aggtggtttt tttatttata aattattcgt ttgatttcgc tttcgataga acaatcaaat

      481 cgtttctgag acgttttagc gtttatttcg tttagttatc ggcataatcg ttaaaacagg

      541 cgttatcgta gcgtaaaagc ccttgagcgt agcgtggctt tgcagcgaag atgttgtctg

      601 ttagattatg aaagccgatg actgaatgaa ataataagcg cagcgtcctt ctatttcggt

      661 tggaggaggc tcaagggagt ttgagggaat gaaattccct catgggtttg attttaaaaa

      721 ttgcttgcaa ttttgccgag cggtagcgct ggaaaatttt tgaaaaaaat ttggaatttg

      781 gaaaaaaatg gggggaaagg aagcgaattt tgcttccgta ctacgacccc ccattaagtg

      841 ccgagtgcca atttttgtgc caaaaacgct ctatcccaac tggctcaagg gtttgagggg

      901 tttttcaatc gccaacgaat cgccaacgtt ttcgccaacg ttttttataa atctatattt

      961 aagtagcttt atttttgttt ttatgattac aaagtgatac actaatttta taaaattatt

     1021 tgattggagt tttttaaatg gtgatttcag aatcgaaaaa aagagttatg atttctctga

     1081 caaaagagca agataaaaaa ttaacagata tggcgaaaca aaaagatttt tcaaaatctg

     1141 cggttgcggc gttagctata gaagaatatg caagaaagga atcagaacaa aaaaaataag

     1201 cgaaagctcg cgtttttaga aggatacgag ttttcgctac ttgtttttga taaggtaatt

     1261 atatcatggc tattaaaaat actaaagcta gaaattttgg atttttatta tatcctgact

     1321 caattcctaa tgattggaaa gaaaaattag agagtttggg cgtatctatg gctgtcagtc

     1381 ctttacacga tatggacgaa aaaaaagata aagatacatg gaatagtagt gatgttatac

     1441 gaaatggaaa gcactataaa aaaccacact atcacgttat atatattgca cgaaatcctg

     1501 taacaataga aagcgttagg aacaagatta agcgaaaatt ggggaatagt tcagttgctc

     1561 atgttgagat acttgattat atcaaaggtt catatgaata tttgactcat gaatcaaagg

     1621 acgctattgc taagaataaa catatatacg acaaaaaaga tattttgaac attaatgatt

     1681 ttgatattga ccgctatata acacttgatg aaagccaaaa aagagaattg aagaatttac

     1741 ttttagatat agtggatgac tataatttgg taaatacaaa agatttaatg gcttttattc

     1801 gccttagggg agcggagttt ggaattttaa atacgaatga tgtaaaagat attgtttcaa

     1861 caaactctag cgcctttaga ttatggtttg agggcaatta tcagtgtgga tatagagcaa

     1921 gttatgcaaa ggttcttgat gctgaaacgg gggaaataaa atgacaaaca aagaaaaaga

     1981 gttatttgct gaaaatgagg aattaaaaaa agaaattaag gacttaaaag agcgtattga

     2041 aagatacaga gaaatggaag ttgaattaag tacaacaata gatttattga gaggagggat

     2101 tattgaataa ataaaagccc ccctgacgaa agtcgacggc aatagttacc cttattatca

     2161 agataagaaa gaaaaggatt tttcgctacg ctcaaatcct ttaaaaaaac acaaaagacc

     2221 acatttttta atgtggtctt ttattcttca actaaagcac ccattagttc aacaaacgaa

     2281 aattggataa agtgggatat ttttaaaata tatatttatg ttacagtaat attgactttt

     2341 aaaaaaggat tgattctaat gaagaaagca gacaagtaag cctcctaaat tcactttaga

     2401 taaaaattta ggaggcatat caaatgaact ttaataaaat tgatttagac aattggaaga

     2461 gaaaagagat atttaatcat tatttgaacc aacaaacgac ttttagtata accacagaaa

     2521 ttgatattag tgttttatac cgaaacataa aacaagaagg atataaattt taccctgcat

     2581 ttattttctt agtgacaagg gtgataaact caaatacagc ttttagaact ggttacaata

     2641 gcgacggaga gttaggttat tgggataagt tagagccact ttatacaatt tttgatggtg

     2701 tatctaaaac attctctggt atttggactc ctgtaaagaa tgacttcaaa gagttttatg

     2761 atttatacct ttctgatgta gagaaatata atggttcggg gaaattgttt cccaaaacac

     2821 ctatacctga aaatgctttt tctctttcta ttattccttg gacttcattt actgggttta

     2881 acttaaatat caataataat agtaattacc ttctacccat tattacagca ggaaaattca

     2941 ttaataaagg taattcaata tatttaccgc tatctttaca ggtacatcat tctgtttgtg

     3001 atggttatca tgctggattg tttatgaact ctattcagga attgtcagat aggcctaatg

     3061 actggctttt ataatatgag ataatgccga ctgtactttt tacagtcggt tttctaatgt

     3121 cactaacctg ccccgttagt tgaagaaggt ttttatatta cagctcc

//
 

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pNZ8148,Plasmid pNZ8148,pNZ8148 vector

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