pOJ260
PVT11373 Packing 2ug
pOJ260 Information
Prokaryotic resistance: amphotericin Apr
Clone strain: Escherichia coli DH5 alpha
Culture conditions: 37 C
pOJ260 Description
POJ260 is a Streptomyces gene knockout expression plasmid.
pOJ260 Sequence
LOCUS Exported 3468 bp ds-DNA circular SYN 13-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..3468
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(169..507)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MITNSISRAAADPLESTCSPSLALAVVLQRRDWENPGVTQLNRLA
AHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRINSPMSSTSGIG
SAADAKCR"
misc_feature 443..499
/label=MCS
promoter complement(556..586)
/label=lac promoter
/note="promoter for the E. coli lac operon"
rep_origin complement(910..1498)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 1702..2502
/codon_start=1
/gene="aac(3)IV"
/product="aminoglycoside 3-N-acetyltransferase type IV
"
/label=ApmR
/note="confers resistance to apramycin (Paget and Davies,
1996)"
/translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
oriT 2947..3056
/note="incP origin of transfer"
ORIGIN
1 atctacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
61 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
121 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
181 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta tgcggtgtga
241 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
301 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
361 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
421 ttgtaaaacg acggccagtg ccaagcttgg gctgcaggtc gactctagag gatccgcggc
481 cgcgcgcgat atcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
541 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
661 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
721 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
781 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
841 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
901 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
961 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
1021 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
1081 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
1141 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
1201 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
1261 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
1321 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
1381 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
1441 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
1501 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
1561 gattttggtc atgagattat caaaaaggat cttcacctag atccttttgg ttcatgtgca
1621 gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt
1681 tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa
1741 tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg
1801 ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg
1861 atcgaggccc tgcgtgctgc gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca
1921 ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt
1981 gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt
2041 gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccacctcac
2101 tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc
2161 gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat
2221 ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc
2281 gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc
2341 cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt
2401 gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag
2461 gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga
2521 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg tggagcggat
2581 cggggattgt ctttcttcag ctcgctgatg atatgctgac gctcaatgcc gtttggcctc
2641 cgactaacga aaatcccgca tttggacggc tgatccgatt ggcacggcgg acggcgaatg
2701 gcggagcaga cgctcgtccg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
2761 tatcgatggt accatatatt taaagatcca ctagtatgca ggtcgacgga tcttttccgc
2821 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc
2881 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg
2941 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt
3001 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggcatg
3061 agcctgtcgg cctacctgct ggccgtcggc cagggctaca aaatcacggg cgtcgtggac
3121 tatgagcacg tccgcgagct ggcccgcatc aatggcgacc tgggccgcct gggcggcctg
3181 ctgaaactct ggctcaccga cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc
3241 gccctgctgg cgaagatcga agagaagcag gacgagcttg gcaaggtcat gatgggcgtg
3301 gtccgcccga gggcagagcc atgacttttt tagccgctaa acggccgggg ggtgcgcgtg
3361 attgccaagc acgtccccat gcgctccatc aagaagagcg acttcgcgga gctggtgaag
3421 tacatcaccg acgagcaagg caagaccgat ccccggggac ctgcatag
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