pOTENT-CMV-Flag-His-Puro
Search name
pOTENT-CMV-Flag-His-Puro,Plasmid pOTENT-CMV-Flag-His-Puro,pOTENT-CMV-Flag-His-Puro vector
pOTENT-CMV-Flag-His-Puro Information
Promoter: CMV, T7
Replicator: M13 ori, ori, SV40 ori
Terminator: bGH poly (A) signal
Plasmid classification: virus series, lentivirus cloning vector
Plasmid size: 7509bp
Prokaryotic resistance: Kan
Screening markers: Neo
Cloned strain: Stbl3
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, instantaneous expression
5'sequencing primers: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'sequencing primers: primers designed according to sequence
Use:Lentiviral
pOTENT-CMV-Flag-His-Puro Description
pOTENT-CMV-Flag-His-Puro Sequence
LOCUS Exported 7509 bp ds-DNA circular SYN 12-JAN-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pOTENT-CMV-Flag-His-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7509)
AUTHORS Huanghua
TITLE Direct Submission
JOURNAL Exported Thursday, September 8, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..7509
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 21..123
/note="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 211..361
/note="Ad5 Psi"
/note="packaging signal for adenovirus serotype 5"
enhancer 419..722
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 723..926
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 954..972
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1073..1096
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/note="FLAG"
/translation="DYKDDDDK"
CDS 1106..1123
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
polyA_signal 1294..1415
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 2342..2671
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2522..2657
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2729..3328
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="PuroR"
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 3329..3553
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
repeat_region 4464..4566
/note="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
rep_origin complement(4838..5426)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 6258..7052
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
rep_origin complement(7100..7480)
/direction=LEFT
/note="M13 ori"
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 ttcgaattta aattaattaa catcatcaat aatatacctt attttggatt gaagccaata
61 tgataatgag ggggtggagt ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg
121 tagtagtgtg gcggaagtgt gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg
181 tggcaaaagt gacgtttttg gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg
241 gttttaggcg gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc
301 gggaaaactg aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa
361 tactgtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa