pPB-CAG-empty-pgk-hph
Catalog No. PVT10887
Packing 2ug
pPB-CAG-empty-pgk-hph Informaiton
Function Mammal Editing plasmids
Prokaryotic resistance: ampicillin Amp
Screening markers: hygromycin Hyg
Cloned strain: Escherichia coli DH5 alpha
Culture conditions: 37 degrees centigrade
pPB-CAG-empty-pgk-hph Description
PPB-CAG-empty-pgk-hph is a transposable expression plasmid of mammalian cells
pPB-CAG-empty-pgk-hph Sequence
> MoCloF 5'-agcgaggaagcggaagagcg-3'
CCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCG
CAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGT
ATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGC
TCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCTTTAACCCTAGAAAGATAATCATATTGTG
ACGTACGTTAAAGATAATCATGCGTAAAATTGACGCATGTGTTTTATCGGTCTGTATATCGAGGTTTATT
TATTAATTTGAATAGATATTAAGTTTTATTATATTTACACTTACATACTAATAATAAATTCAACAAACAA
TTTATTTATGTTTATTTATTTATTAAAAAAAAACAAAAACTCAAAATTTCTTCTATAAAGTAACAAAACA
TAACTTCGTATAGCATACATTATACGAAGTTATCTCGACGCGGCCGCTCTAGCCTCGAGGCTAGAACTAG
TGGATCTCGAGCCCCAGCTGGTTCTTTCCGCCTCAGAAGCCATAGAGCCCACCGCATCCCCAGCATGCCT
GCTATTGTCTTCCCAATCCTCCCCCTTGCTGTCCTGCCCCACCCCACCCCCCAGAATAGAATGACACCTA
CTCAGACAATGCGATGCAATTTCCTCATTTTATTAGGAAAGGACAGTGGGAGTGGCACCTTCCAGGGTCA
AGGAAGGCACGGGGGAGGGGCAAACAACAGATGGCTGGCAACTAGAA
> F1ori-R 5'-AGGGAAGAAAGCGAAAGGAG-3'
GTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCgCGTCCCATT
CGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGC
GAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAA
ACGACGGCCAGTGAATTGTAATACGACTCACTATAGGGCGAATTGGGTACCTTAACCCTAGAAAGATAGT
CTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTCTCTCTTTCTAAATAGCGCGAATCCGTCGCTG
TGCATTTAGGACATCTCAGTCGCCGCTTGGAGCTCCCGTGAGGCGTGCTTGTCAATGCGGTAAGTGTCAC
TGATTTTGAACTATAACGACCGCGTGAGTCAAAATGACGCATGATTATCTTTTACGTGACTTTTAAGATT
TAACTCATACGATAATTATATTGTTATTTCATGTTCTACTTACGTGATAACTTATTATATATATATTTTC
TTGTTATAGATATCCAACTATGAAACATTATCATAAAGCAATAGTAAAGAGCCTTCAGTAAAGAGCAGGC
ATTTATCTAATCCCACCCCACCCCCACCCCCGTAGCTCCAATCCTTCCATTCAAAATGTAGGTACTCTGT
TCTCACCCTTCTTAACAAAGTATGACAGGAAAAACTTCCATGTGGACATCTTTATTGTTTAATAGATCAT
CAATTTCTCGACTATTCCTTTGCCCTCGGACGAGTGCTGGGGCGTCGGTTTCCACTATCGGCGAGTACTT
CTACACAGCCATCGGTCCA
