pPD95- 67 Plasmid

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  • Model: PVT5402
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PPD95_67

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pPD95-67,Plasmid pPD95-67,pPD95-67 vector

 

 

PPD95_67 Informaiton

Replicon: pUC ori

Plasmid classification: nematode expression plasmid

Plasmid size: 4532bp

Plasmid tagging: IVSA, SV40NLS, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSL, decoy

Prokaryotic resistance: ampicillin Ampicillin

Clonal strain: DH5 alpha

Culture conditions: 37 C, aerobic, LB

Expression host: nematode

Culture conditions: refer to relevant literature.

Ways of induction: referring to relevant literature

5'sequencing primers: M13R:CAGGAAACAGCTATGACC

3'sequencing primers: Based on full sequence design

 

PPD95_67 Description

PPD95_67  is a nematode expression plasmid with GFP tag.

 

 

PPD95_67 Multiple cloning site

pPD95_67-vector

 

 

PPD95_67 Sequence

LOCUS       Exported                4532 bp ds-DNA     circular SYN 04-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pPD95_67
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, September 4, 2016 from SnapGene Viewer 3.1.4
           
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             146..166
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     misc_feature    167..580
                     /note="GFP"
     promoter        2277..2381
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2382..3242
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3413..4001
                     /direction=RIGHT
                     /note="pUC ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4289..4310
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4325..4355
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4363..4379
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4387..4403
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggagggt
      121 accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg tagaaaaaat
      181 gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      241 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
      301 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa
      361 catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt
      421 ctgttatggt gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt
      481 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      541 gaactacaag acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
      601 ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      661 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      721 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa
      781 catgatttta ctaactaact aatctgattt aaattttcag aacttcaaaa ttagacacaa
      841 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
      901 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
      961 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     1021 acatggcatg gatgaactat acaaatagca ttcgtagaat tccaactgag cgccggtcgc
     1081 taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt
     1141 taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc gcgcccgtgc
     1201 ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc
     1261 ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc
     1321 ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
     1381 aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
     1441 cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc
     1501 atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa
     1561 tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa
     1621 gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg
     1681 cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt
     1741 gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt
     1801 gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt gtctatgggg
     1861 tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt ttcctactag
     1921 tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     1981 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     2041 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     2101 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     2161 gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt ttataggtta
     2221 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     2281 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2341 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2401 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2461 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
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