pPR3-C
Catalog No. PVT11261
Packing 2ug
pPR3-C Information
Function yeast Hybrid plasmid
Promoter: ADH1 promoter
Replicator: pUC ori, F1 ori, 2 mu ori
Terminator: CYC1 Terminator
Plasmid classification: yeast cell plasmids; yeast hybrid plasmids; two hybrid plasmids
Plasmid size: 6620bp
Plasmid label: C-HA, C-NubG
Prokaryotic resistance: ampicillin Amp
Screening markers: TRP1
Cloned strains of Escherichia coli, DH5 A and other Escherichia coli
Culture conditions: 37 centigrade, LB, aerobic
Expression host: yeast
Culture conditions: 30 centigrade, YPD, aerobic
Note: high copy plasmids
pPR3-C Description
PPR3-C is a plasmid for yeast two hybrid experiment of membrane proteins.
The DUALmembrane system is intended for the detection and identification of interactions involving integral membrane proteins and membrane-associated proteins in yeast. The DUALmembrane pairwise interaction kit enables you to:
Investigate the interaction between a membrane protein (integral or membrane-associated) and a membrane protein or soluble protein
Map domains or amino acids which are critical for an interaction
Screen cDNA libraries using a membrane protein as a bait to find novel
interacting proteins (requires purchase of a separate cDNA library from Dualsystems)