pProEX HTA

PVT0373   2ug
 

pProEX HTA Infomation

Plasmid type: expression of Escherichia coli

Expression level: high

Cloning methods: polyclonal sites, restrictive endonucleases

Carrier size: 4751bp

Sequence primer sequence of 5'sequencing: M13/pUC Reverse: AGCGGATAACAATTTCACACAGG (Invitrogen)

Sequence primer sequence of 3'sequencing: pTrcHis Reverse: GATTTAATCTGTATCAGG (Invitrogen)

Carrier label: N-His, N-TEV protease cutting site

Carrier resistance: ampicin

Remarks:

Hosts: E.coli. Related vectors: pBR322.

Product directory number: 50609

Stability: instantaneous expression of Transient

Composition / inducible type: inducible type

Virus / non virus: non virus
 

pProEX HTA Description
pProEX HT series prokaryotic expression system is a carrier used to express foreign proteins in Escherichia coli. The target protein expressed by this carrier contains 6 His purify labels. The target gene can be cloned into the polyclonal site of the vector. After the expression of pProEX HTa, B and C. protein is selected, the His purification tag is located at the N end of the target protein. Meanwhile, the use of His purification tag can be used for protein purification. The rTEV protease identification site is also contained on the carrier, and the rTEV protease can be used for enzyme digestion to remove the fused His label.

Reference for PVT0373 pProEX HTA
1. Article: Identification of protective peptides of Fasciola hepatica-derived cathepsin L1 (FhCL1) in vaccinated sheep by a linear B-cell epitope mapping approach
Author:Leandro Buffoni, Laura Garza-Cuartero, Raúl Pérez-Caballero, Rafael Zafra, F. Javier Martínez-Moreno, Verónica Molina-Hernández, José Pérez, Álvaro Martínez-Moreno & Grace Mulcahy
Parasites & Vectors volume 13, Article number: 390 (2020) 
DOI
https://doi.org/10.1186/s13071-020-04260-6

2. Article: Efficacy of a multivalent vaccine against Fasciola hepatica infection in sheep

Authors:Rafael Zafra, Leandro Buffoni, Raúl Pérez-Caballero, Verónica Molina-Hernández, María T. Ruiz-Campillo, José Pérez, álvaro Martínez-Moreno & Francisco J. Martínez Moreno 

Veterinary Research volume 52, Article number: 13 (2021)
Published: 28 January 2021

pProEX HTA Sequence

LOCUS       pPROEX HTa    4751 bp     DNA    circular     SYN
DEFINITION  pPROEX HTa
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="pPROEX HTa"
                     /mol_type="other DNA"
     promoter        193..266
                     /label="trc_promoter"
     misc_feature    235..257
                     /label="M13_pUC_rev_primer"
     misc_feature    complement(472..489)
                     /label="pTrcHis_rev_primer"
     misc_feature    complement(472..489)
                     /label="pBAD_rev_primer"
     terminator      522..679
                     /label="rrnB_terminator"
     terminator      645..688
                     /label="rrnB_T1_terminator"
     terminator      820..847
                     /label="rrnB_T2_terminator"
     promoter        889..917
                     /label="AmpR_promoter"
     gene            959..1819
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             959..1819
                     /label="ORF frame 2"
     rep_origin      1974..2593
                     /label="pBR322_origin"
     rep_origin      complement(2931..3237)
                     /label="f1_origin"
     misc_feature    3452..3474
                     /label="pGEX_3_primer"
     misc_feature    3621..4712
                     /label="lacI"
     CDS             3753..4712
                     /label="ORF frame 3"
ORIGIN
    1 GTTTGACAGC TTATCATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC
   61 GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC
  121 GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC
  181 TGAAATGAGC TGTTGACAAT TAATCATCCG GTCCGTATAA TCTGTGGAAT TGTGAGCGGA
  241 TAACAATTTC ACACAGGAAA CAGACCATGT CGTACTACCA TCACCATCAC CATCACGATT
  301 ACGATATCCC AACGACCGAA AACCTGTATT TTCAGGGCGC CATGGATCCG GAATTCAAAG
  361 GCCTACGTCG ACGAGCTCAA CTAGTGCGGC CGCTTTCGAA TCTAGAGCCT GCAGTCTCGA
  421 GGCATGCGGT ACCAAGCTTG GCTGTTTTGG CGGATGAGAG AAGATTTTCA GCCTGATACA
  481 GATTAAATCA GAACGCAGAA GCGGTCTGAT AAAACAGAAT TTGCCTGGCG GCAGTAGCGC
  541 GGTGGTCCCA CCTGACCCCA TGCCGAACTC AGAAGTGAAA CGCCGTAGCG CCGATGGTAG
  601 TGTGGGGTCT CCCCATGCGA GAGTAGGGAA CTGCCAGGCA TCAAATAAAA CGAAAGGCTC
  661 AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA
  721 GGACAAATCC GCCGGGAGCG GATTTGAACG TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG
  781 CAGGACGCCC GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG
  841 GCCTTTTTGC GTTTCTACAA ACTCTTTTTG TTTATTTTTC TAAATACATT CAAATATGTA
  901 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT
  961 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT
 1021 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG
 1081 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA
 1141 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG
 1201 TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT
 1261 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG
 1321 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG
 1381 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA
 1441 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC
 1501 TACAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC
 1561 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC
 1621 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG
 1681 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC
 1741 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC
 1801 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT
 1861 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC
 1921 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA
 1981 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC
 2041 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT
 2101 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG
 2161 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC
 2221 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT
 2281 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA
 2341 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT
 2401 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG
 2461 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA
 2521 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA
 2581 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT
 2641 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
 2701 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
 2761 GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATAATTTT
 2821 GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT
 2881 CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT
 2941 TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT
 3001 CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG
 3061 GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG
 3121 AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC
 3181 GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC
 3241 GCTACAGGGC GCGTCCCATT CGCCATTCAG GCTGCTATGG TGCACTCTCA GTACAATCTG
 3301 CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG
 3361 GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG
 3421 GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA
 3481 CCGTCATCAC CGAAACGCGC GAGGCAGCAG ATCAATTCGC GCGCGAAGGC GAAGCGGCAT
 3541 GCATTTACGT TGACACCATC GAATGGTGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC
 3601 CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG
 3661 AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT
 3721 CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC
 3781 GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC
 3841 TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG
 3901 GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG
 3961 TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC
 4021 AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT
 4081 CTGACCAGAC ACCCATCAAC AGTATTATTT TCTCCCATGA AGACGGTACG CGACTGGGCG
 4141 TGGAGCATCT GGTCGCATTG GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT
 4201 CTGTCTCGGC GCGTCTGCGT CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC
 4261 AGCCGATAGC GGAACGGGAA GGCGACTGGA GTGCCATGTC CGGTTTTCAA CAAACCATGC
 4321 AAATGCTGAA TGAGGGCATC GTTCCCACTG CGATGCTGGT TGCCAACGAT CAGATGGCGC
 4381 TGGGCGCAAT GCGCGCCATT ACCGAGTCCG GGCTGCGCGT TGGTGCGGAT ATCTCGGTAG
 4441 TGGGATACGA CGATACCGAA GACAGCTCAT GTTATATCCC GCCGTTAACC ACCATCAAAC
 4501 AGGATTTTCG CCTGCTGGGG CAAACCAGCG TGGACCGCTT GCTGCAACTC TCTCAGGGCC
 4561 AGGCGGTGAA GGGCAATCAG CTGTTGCCCG TCTCACTGGT GAAAAGAAAA ACCACCCTGG
 4621 CACCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC
 4681 GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCGC
 4741 GAATTGATCT G
//

Caution:
1.  This product is FOR RESEARCH USE ONLY!
2.  The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.

Search name

pProEX HTA,Plasmid pProEX HTA,pProEX HTA vector