pProEX HTC
PVT0375 2ug
pProEX HTC Information
Promoter: T7/lac
Replicator: ColE1 ori, F1 ori
Terminator: rrnB T1 Terminator
Plasmid classification: Escherichia coli vector; PET series expression plasmid
Plasmid size: 4779bp
Plasmid label: N-6 x His, N-TEV
Prokaryotic resistance: ampicillin Amp
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: BL21 (DE3)
Culture conditions: 37 centigrade, aerobic, LB
Inducement: IPTG or lactose and its analogues
5'sequencing primers: T7:TAATACGACTCACTATAGGG
pProEX HTC Decription
The prokaryotic expression system of pProEX HT series is used to express foreign proteins in E. coli. The target protein expressed by this vector contains 6 His purification labels. The target gene can be cloned to the polyclonal loci of the carrier. After the expression of pProEX HTa, B, C. protein, the His purification label is located at the N end of the target protein, and the use of His purification label can be used for protein purification. The vector contains rTEV protease recognition sites, which can be digested by rTEV protease, thus removing the fused His tag.
pProEX HTC Sequence
LOCUS Exported 4779 bp ds-DNA circular SYN 27-8-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4779)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-8-27
FEATURES Location/Qualifiers
source 1..4779
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 230..246
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 279..296
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 318..338
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/note="TEV Site"
/translation="ENLYFQG"
terminator 644..730
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 822..849
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 869..960
/gene="bla"
/note="AmpR promoter"
CDS 961..1821
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1992..2580
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
rep_origin complement(2828..3256)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3581..3658
/gene="lacI (mutant)"
/note="lacIq promoter"
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 3659..4741
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
ORIGIN
1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
181 tgaaatgagc tgttgacaat taatcatccg gtccgtataa tctgtggaat tgtgagcgga
241 taacaatttc acacaggaaa cagaccatgt cgtactacca tcaccatcac catcacgatt
301 acgatatccc aacgaccgaa aacctgtatt ttcagggcgc catggggatc cggaattcaa
361 aggcctacgt cgacgagctc actagtcgcg gccgctttcg aatctagagc ctgcagtctc
421 gaggcatgcg gtaccaagct tggctgtttt ggcggatgag agaagatttt cagcctgata
481 cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc
541 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt
601 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
661 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
721 taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg
781 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga
841 tggccttttt gcgtttctac aaactctttt tgtttatttt tctaaataca ttcaaatatg
901 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
961 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
1021 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
1081 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
1141 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
1201 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
1261 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
1321 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
1381 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
1441 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
1501 cctacagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
1561 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
1621 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
1681 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
1741 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
1801 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
1861 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
1921 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
1981 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
2041 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
2101 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
2161 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
2221 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
2281 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
2341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
2401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
2461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
2521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
2581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
2641 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
2701 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
2761 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataatt
2821 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
2881 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
2941 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
3001 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
3061 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
3121 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
3181 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
3241 ccgctacagg gcgcgtccca ttcgccattc aggctgctat ggtgcactct cagtacaatc
3301 tgctctgatg ccgcatagtt aagccagtat acactccaat attgctacgt gactggtaca
3361 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
3421 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
3481 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaga
3541 tcaattcgcg cgcgaaggcg aagcggcatg catttacgtt gacaccatcg aatggtgcaa
3601 aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt
3661 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc
3721 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc
3781 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc
3841 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc
3901 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg
3961 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg
4021 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac
4081 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt
4141 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca
4201 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg
4261 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag
4321 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc
4381 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg
4441 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg
4501 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
4561 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
4621 ctcactggtg aaaagaaaaa ccaccctggc acccaatacg caaaccgcct ctccccgcgc
4681 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
4741 agcgcaacgc aattaatgtg agttagcgcg aattgatct
//
Caution:
Product is for research use only!
Search name
pProEX HTC,Plasmid pProEX HTC,pProEX HTC vector
