pQE-31
PVT0523 2ug
pQE-31 Information
Replicon: Ori
Terminator: Lambda t0
Plasmid classification: Escherichia coli vector; pQE series expression plasmid.
Plasmid size: 3463bp
Plasmid label: N-His
Prokaryotic resistance: ampicillin Amp
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: M15
Culture conditions: 37 centigrade, aerobic, LB
Induction mode: no induction
5'sequencing primers: pQE30-F: AGCGGATAACAATTTCACACAG
3'sequencing primers: pQE30-R: TTCTGAGGTCATTACTGGATC
pQE-31 Description
pQE-31 Sequence
LOCUS Exported 3463 bp ds-DNA circular SYN 08-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS PQE-31
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3463)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, September 8, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..3463
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 97..108
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 127..144
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 210..304
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 348..1007
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1072..1158
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 1313..1453
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(1639..2227)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2398..3258)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3259..3363)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
121 ggatctcacc atcaccatca ccatacggat ccgcatgcga gctcggtacc ccgggtcgac
181 ctgcagccaa gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc
241 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
301 gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
361 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
421 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
481 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
541 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
601 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
661 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
721 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
781 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
841 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
901 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
961 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
1021 gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa
1081 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
1141 ctcctgagta ggacaaatcc gccctctaga gctgcctcgc gcgtttcggt gatgacggtg
1201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
1261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
1321 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
1381 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
1441 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
1501 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
1561 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
1621 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
1681 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
1741 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
1801 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
1861 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
1921 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
1981 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
2041 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
2101 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
2161 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
2221 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
2281 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
2341 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
2401 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
2461 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
2521 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
2581 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
2641 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
2701 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
2761 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
2821 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
2881 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
2941 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
3001 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
3061 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
3121 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
3181 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
3241 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
3301 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
3361 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
3421 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cac
//
Caution
Product is for research use only!
Search name
pQE-31,Plasmid pQE-31,pQE-31 vector
