pQE-80L
PVT0534 2ug
pQE-80L Information
Replicon: Ori
Terminator: Lambda t0
Plasmid classification: Escherichia coli vector; pQE series expression plasmid
Plasmid size: 4751bp
Plasmid Tags: 6*His
Prokaryotic resistance: ampicillin Amp
Clone strain: DH5 alpha
Culture conditions: 37, aerobic, LB
Expression host: M15
Culture conditions: 37, aerobic, LB
Induction method: no need to induce
Primers for 5'sequencing: pQE30-F: AGCGGATAACAATTTCACACAG
Primers for 3'sequencing: pQE30-R: TTCTGAGGTCATTACTGGATC
pQE-80L Description
pQE-80L is a protein expression plasmid of Escherichia coli.
pQE-80L Sequence
LOCUS Exported File 4751 bp ds-DNA circular SYN 01-2-2015
KEYWORDS Untitled 4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4751)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015-2-1
FEATURES Location/Qualifiers
source 1..4751
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 127..144
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 208..302
/gene="
"
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 346..1005
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1070..1156
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(1249..2331)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(2332..2409)
/gene="lacI (mutant)"
/note="lacIq promoter"
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
misc_feature 2601..2741
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(2927..3515)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3686..4546)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4547..4651)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
121 ggatcgcatc accatcacca tcacggatcc gcatgcgagc tcggtacccc gggtcgacct
181 gcagccaagc ttaattagct gagcttggac tcctgttgat agatccagta atgacctcag
241 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
301 atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
361 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
421 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
481 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
541 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg
601 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
661 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
721 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
781 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
841 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
901 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
961 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
1021 tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg
1081 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
1141 cctgagtagg acaaatccgc cctctagatt acgtgcagtc gatgataagc tgtcaaacat
1201 gagaattgtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
1261 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
1321 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
1381 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
1441 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
1501 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt
1561 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
1621 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
1681 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
1741 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
1801 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
1861 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
1921 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
1981 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
2041 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
2101 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
2161 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
2221 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
2281 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
2341 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcaccattc
2401 gatggtgtcg gaatttcggg cagcgttggg tcctggccac gggtgcgcat gatctagagc
2461 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
2521 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
2581 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
2641 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
2701 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
2761 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
2821 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
2881 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
2941 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
3001 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
3061 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
3121 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
3181 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
3241 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
3301 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
3361 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
3421 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
3481 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
3541 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
3601 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
3661 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
3721 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
3781 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
3841 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
3901 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
3961 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
4021 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
4081 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
4141 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
4201 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
4261 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
4321 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
4381 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
4441 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
4501 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
4561 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
4621 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
4681 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
4741 ttcgtcttca c
//
Caution:
Product is for research use only!
Search name
pQE-80L,Plasmid pQE-80L,pQE-80L vector