pQE-60 Plasmid
PVT0531 2ug
pQE-60 Plasmid Information
Promoter: T5
Plasmid classification: Escherichia coli vector; pQE series expression plasmid
Plasmid size: 3431bp
Plasmid label: C-His
Prokaryotic resistance: ampicillin Amp
Cloned strain: Escherichia coli DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: Escherichia coli M15
Culture conditions: 37 centigrade, aerobic, LB
5'sequencing primers: pQE30-F: AGCGGATAACAATTTCACACAG
3'sequencing primers: pQE30-R: TTCTGAGGTCATTACTGGATC
pQE-60 Plasmid Description
PQE-60 is a pQE series expression plasmid of Escherichia coli.
pQE-60 Plasmid Sequence
LOCUS Exported 3431 bp ds-DNA circular SYN 08-SEP-2016
DEFINITION synthetic circular DNA
KEYWORDS PQE-60
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3431)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3431
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 97..108
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 133..150
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 173..267
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
terminator 1039..1125
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 1281..1421
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(1607..2195)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2366..3226)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3227..3331)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatggga
121 ggatccagat ctcatcacca tcaccatcac taagcttaat tagctgagct tggactcctg
181 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc
241 cgccgggcgt tttttattgg tgagaatcca agctagcttg gcgagatttt caggagctaa
301 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
361 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
421 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
481 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat
541 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
601 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
661 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
721 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
781 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgc atgggcaaat
841 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct
901 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
961 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggggtaatga
1021 ctctctagct tgaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
1081 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gctctagagc
1141 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
1201 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
1261 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
1321 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
1381 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
1441 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
1501 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
1561 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
1621 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
1681 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
1741 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
1801 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
1861 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
1921 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
1981 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
2041 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
2101 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
2161 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
2221 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
2281 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
2341 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
2401 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
2461 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
2521 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
2581 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
2641 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
2701 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
2761 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
2821 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
2881 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
2941 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
3001 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
3061 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
3121 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
3181 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
3241 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
3301 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
3361 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
3421 ttcgtcttca c
//
Search name
pQE-60,Plasmid pQE-60,pQE-60 vector
