pRRLSIN.CPPT.PGK-GFP.WPRE
Search name
pRRLSIN.CPPT.PGK-GFP.WPRE,Plasmid pRRLSIN.CPPT.PGK-GFP.WPRE,pRRLSIN.CPPT.PGK-GFP.WPRE vector
pRRLSIN.CPPT.PGK-GFP.WPRE plasmid infomation
Promoters: hPGK, T3, T7
Replicon: pUC ori, F1 ori, SV40 ori
Terminator: SV40 poly (A) signal
Plasmid classification: virus series, lentiviral cloning vector
Prokaryotic resistance: Amp
Clone strain: Stbl3
Culture conditions: 37 LB, aerobic
Expression host: mammalian cells
Induction method: no induction, transient expression
Primers for 5'sequencing: pEGFP-C-5:CATGGTCCTGCTGGAGTTCGTG
Primers for 3'sequencing: primers were designed according to the sequence
Use:Lentiviral
pRRLSIN.CPPT.PGK-GFP.WPRE plasmid sequence
LOCUS Exported 8931 bp ds-DNA circular SYN 23-JUN-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pRRLSIN.cPPT.PGK-GFP.WPRE
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8931)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Friday, June 23, 2017 from SnapGene Viewer 3.3.4
http://www.maiolingbio.com
FEATURES Location/Qualifiers
source 1..8931
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..227
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 228..408
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 455..580
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1073..1306
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 3345..3462
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 3508..4018
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
CDS 4040..4759
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 4775..5363
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(5246..5257)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5450..5683
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5755..5876
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 5916..6051
/label=SV40 ori
/note="SV40 origin of replication"
promoter complement(6072..6090)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6100..6116)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 6258..6713
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6739..6843
/gene="bla"
/label=AmpR promoter
CDS 6844..7704
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7875..8463
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 8751..8772
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8787..8817
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8825..8841
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8849..8865
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 8886..8904
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
