pRSV- Rev

$193.00

  • Model: PVT11130
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pRSV-Rev

Catalog No. PVT11130
Packing 2ug

 

pRSV-Rev Information

Promoter: RSV

Replicator: pUC ori, F1 ori

Plasmid classification: virus series, lentivirus packaging carrier

Plasmid size: 4180bp

Prokaryotic resistance: Amp

Cloned strain: Stbl3

Culture conditions: 37 centigrade, aerobic LB

Expression host: mammalian cells

Induction mode: no induction, instantaneous expression

5'sequencing primers: M13R:CAGGAAACAGCTATGACC

3'sequencing primers: primers designed according to sequence

 

 

pRSV-Rev Description

The HIV-1 Rev of the second and third exons added to the Rev cDNA expression plasmid is controlled by the transcription of the RSV U3 promoter. Note that most trono lab lentivirus vectors can only use the second generation packaging system.

pRSV-Rev

 

pRSV-Rev Sequence

LOCUS       Exported                4180 bp ds-DNA     circular SYN 01-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, September 1, 2016 from SnapGene Viewer 3.1.4
             
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1024..1612
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1900..1921
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1936..1966
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1974..1990
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1998..2014
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2159..2420
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2983..2999)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3331..3786
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4068..4172
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             4173..853
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
       61 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      121 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
      181 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
      241 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      301 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      361 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      421 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      481 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
      541 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
      601 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
      661 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
      721 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
      781 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
      841 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
      901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
      961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1141 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1681 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     1741 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     1801 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1861 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1921 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1981 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga tgtacgggcc
     2041 agatatacgc gtatctgagg ggactagggt gtgtttaggc gaaaagcggg gcttcggttg
     2101 tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa
     2161 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
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