pSAT6-nEYFP-N1
Catalog No. PVT11205
Packing 2ug
pSAT6-nEYFP-N1 Information
Promoter: 2X35S promoter + TL enhancer
Replicon: pUC ori
Terminator: 35S Terminator
Plasmid Classification: Plant Series Plasmids; Plant Reporting Plasmids; Bipartite Fluorescent Plasmids
Plasmid size: 4417 BP
Plasmid label: C-n(1-174) EYFP
Prokaryotic resistance: ampicillin Amp
Screening Markers: None
Cloning strains: Escherichia coli such as DH5alpha
Culture conditions: 37 C, aerobic, LB
Expression host: plant
Induction: Constitutive expression without induction
5'Sequencing Primers: 35S: GACGCAATCCCACTATCC
3'Sequencing primers: 35S-ter: CTCAACATGAGCGAAACCC
pSAT6-nEYFP-N1 Description
The pSAT6-nEYFP-N1 plasmid is a vector for plant bimolecular fluorescence experiments. 2X35S promoter + TL enhancer drives the expression of EYFP.
pSAT6-nEYFP-N1 Sequence
LOCUS Exported 4417 bp ds-DNA circular SYN 25-AUG-2017
DEFINITION synthetic circular DNA
KEYWORDS pSAT6-nEYFP-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4417)
AUTHORS NCBI Entrez
TITLE Direct Submission
JOURNAL Exported Aug 25, 2017 from Nova lifetech
FEATURES Location/Qualifiers
source 1..4417
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature complement(149..398)
/label=ALPHA
/note="/vntifkey=21"
misc_feature complement(399..428)
/label=PI-PspI
/note="/vntifkey=21"
misc_feature 429..1320
/label=2X35S promoter + TL enhancer
/label=2X35Spromoter+TLenhancer
/label=nonstandard type: misc_marker
/note="/vntifkey=22"
primer_bind 1110..1129
/label=35S
misc_feature 1323..1394
/label=MCS
/note="/vntifkey=21"
misc_feature 1395..1919
/label=n(1-174)EYFP
/note="/vntifkey=21"
misc_feature 1938..2156
/label=35S terminator
/label=35Sterminator
/note="/vntifkey=21"
primer_bind complement(2041..2061)
/label=35S-ter
misc_feature 2157..2186
/label=PI-PspI
/note="/vntifkey=21"
misc_feature complement(2598..3186)
/label=pUC ori
misc_feature complement(3360..4217)
/label=Amp
/label=AMP(R)
/note="/vntifkey=21"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag
481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca
781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa
1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta
1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag
1321 ccatggtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg
1381 ggcccgggat cctgatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
1441 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
1501 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
1561 tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc
1621 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
1681 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
1741 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
1801 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
1861 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggactgaa
1921 gatccaccta gtctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct
1981 ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata
2041 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
2101 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgacgc ggccgcaccc
2161 ataataccca taatagctgt ttgccagtaa tcatggtcat agctgtttcc tgtgtgaaat
2221 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
2281 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
2341 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
2401 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
2461 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
2521 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
2581 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
2641 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
2701 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
2761 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
2821 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
2881 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
2941 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
3001 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
3061 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
3121 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
3181 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
3241 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
3301 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
3361 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
3421 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
3481 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
3541 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
3601 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
3661 gttgccattg ctacaggc
...
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.
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