pSAT6- nEYFP- N1

$160.00

  • Model: PVT11205
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pSAT6-nEYFP-N1


Catalog No. PVT11205
Packing 2ug

 

pSAT6-nEYFP-N1 Information

Promoter: 2X35S promoter + TL enhancer

Replicon: pUC ori

Terminator: 35S Terminator

Plasmid Classification: Plant Series Plasmids; Plant Reporting Plasmids; Bipartite Fluorescent Plasmids

Plasmid size: 4417 BP

Plasmid label: C-n(1-174) EYFP

Prokaryotic resistance: ampicillin Amp

Screening Markers: None

Cloning strains: Escherichia coli such as DH5alpha

Culture conditions: 37 C, aerobic, LB

Expression host: plant

Induction: Constitutive expression without induction

5'Sequencing Primers: 35S: GACGCAATCCCACTATCC

3'Sequencing primers: 35S-ter: CTCAACATGAGCGAAACCC

 

pSAT6-nEYFP-N1 Description

The pSAT6-nEYFP-N1 plasmid is a vector for plant bimolecular fluorescence experiments. 2X35S promoter + TL enhancer drives the expression of EYFP.

 

pSAT6-nEYFP-N1 Sequence

LOCUS       Exported                4417 bp ds-DNA     circular SYN 25-AUG-2017
DEFINITION  synthetic circular DNA
KEYWORDS    pSAT6-nEYFP-N1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   NCBI Entrez
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2017 from Nova lifetech
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(149..398)
                     /label=ALPHA
                     /note="/vntifkey=21"
     misc_feature    complement(399..428)
                     /label=PI-PspI
                     /note="/vntifkey=21"
     misc_feature    429..1320
                     /label=2X35S promoter + TL enhancer
                     /label=2X35Spromoter+TLenhancer
                     /label=nonstandard type: misc_marker
                     /note="/vntifkey=22"
     primer_bind     1110..1129
                     /label=35S
     misc_feature    1323..1394
                     /label=MCS
                     /note="/vntifkey=21"
     misc_feature    1395..1919
                     /label=n(1-174)EYFP
                     /note="/vntifkey=21"
     misc_feature    1938..2156
                     /label=35S terminator
                     /label=35Sterminator
                     /note="/vntifkey=21"
     primer_bind     complement(2041..2061)
                     /label=35S-ter
     misc_feature    2157..2186
                     /label=PI-PspI
                     /note="/vntifkey=21"
     misc_feature    complement(2598..3186)
                     /label=pUC ori
     misc_feature    complement(3360..4217)
                     /label=Amp
                     /label=AMP(R)
                     /note="/vntifkey=21"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
      421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag
      481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
      541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
      601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
      661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
      721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca
      781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
      901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
      961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa
     1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta
     1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag
     1321 ccatggtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg
     1381 ggcccgggat cctgatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1441 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1501 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1561 tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc
     1621 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1681 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1741 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1801 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1861 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggactgaa
     1921 gatccaccta gtctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct
     1981 ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata
     2041 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
     2101 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgacgc ggccgcaccc
     2161 ataataccca taatagctgt ttgccagtaa tcatggtcat agctgtttcc tgtgtgaaat
     2221 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2281 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2341 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2401 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2461 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2521 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2581 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2641 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2701 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2761 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     2821 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2881 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2941 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3001 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     3061 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3121 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3181 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3241 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3301 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3361 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3421 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3481 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3541 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3601 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3661 gttgccattg ctacaggc
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