pSilencer 4.1- CMV puro- Stuffer

$228.00

  • Model: PVT10871
  • 50 Units in Stock
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pSilencer 4.1-CMV puro-Stuffer


Catalog No. PVT10871
Packing 2ug
Function Mammal Editing plasmids
Resistance Amp
Screen Puro
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon pUC
Copy high
Promoter
Induction
Forward primer
Reverse primer

 

pSilencer 4.1-CMV puro-Stuffer Description

PSilencer 4.1-CMV puro-Stuffer is a mammalian cell interfering RNA expression plasmid.

 

pSilencer 4.1-CMV puro-Stuffer Sequence

LOCUS       Exported                6679 bp ds-DNA     circular SYN 19-DEC-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1586..1789)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1790..2093
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2685..3273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3444..4304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4305..4409)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4497..4578
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4808..5407)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(5504..5820)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5518..5653
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    6235..6293
                     /label=CMV pA signal
     misc_feature    6299..1564
                     /label=Stuffer
ORIGIN
        1 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
       61 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
      121 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
      181 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
      241 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
      301 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
      361 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
      421 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
      481 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
      541 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
      601 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
      661 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
      721 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
      781 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
      841 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
      901 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
      961 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
     1021 tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg
     1081 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     1141 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     1201 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     1261 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc
     1321 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1381 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1441 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1501 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     1561 ataaggatcc acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc
     1621 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
     1681 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     1741 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
     1801 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     1861 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     1921 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     1981 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     2041 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     2101 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctaagatctg
     2161 gtaccttgaa ttcatgcttc tcctcccttt agtgagggta attctctctc tctccctata
     2221 gtgagtcgta ttaattcctt ctcttctata gtgtcaccta aatcgttgca attcgtaatc
     2281 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     2341 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     2461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     2521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2941 cacgaacccc ccgttcagcc
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