pSilent- 1 Plasmid

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  • Model: PVT5309
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pSilent-1

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pSilent-1,Plasmid pSilent-1,pSilent-1 vector

 

pSilent-1 Information

Promoter: TrpC

Replicator: pUC ori, F1 ori

Terminator: TRPC

Plasmids: filamentous fungal carriers; RNAi gene silencing interference plasmids

Plasmid size: 7056bp

Prokaryotic resistance: ampicillin Ampicillin

Screening markers: hygromycin Hygromycin

Cloned strain: DH5 alpha

Culture conditions: 37 centigrade, aerobic, LB

Expression host: filamentous fungi

5'sequencing primers: Based on sequence design

3'sequencing primers: Based on sequence design

Use:Filamentous fungal plasmid

 

pSilent-1 Description

PSilent-1 plasmid is a carrier for RNAi gene silencing, which has XhoI, SnaBI, HindIII (MCS1) and BglII, SphI, StuI, KpnI, ApaI (MCS2).

pSilent-1-vector

 

pSilent-1 Sequence

Gene silencing vector pSilent-1 DNA, complete sequence
GenBank: AB303070.2
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 LOCUS       AB303070                7056 bp    DNA     circular SYN 11-DEC-2008
DEFINITION  Gene silencing vector pSilent-1 DNA, complete sequence.
ACCESSION   AB303070
VERSION     AB303070.2  GI:217272796
KEYWORDS    .
SOURCE      Gene silencing vector pSilent-1
  ORGANISM  Gene silencing vector pSilent-1
            other sequences; artificial sequences; vectors.
REFERENCE   1
  AUTHORS   Nakayashiki,H., Hanada,S., Nguyen,B.Q., Kadotani,N., Tosa,Y. and
            Mayama,S.
  TITLE     RNA silencing as a tool for exploring gene function in ascomycete
            fungi
  JOURNAL   Fungal Genet. Biol. 42 (4), 275-283 (2005)
   PUBMED   15749047
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Nakayashiki,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2007) Contact:Hitoshi Nakayashiki Kobe
            University, Graduate school of agricultural science; 1 Rokko-dai
            Nada, Kobe 657-8501, Japan
COMMENT     On Dec 10, 2008 this sequence version replaced gi:146455412.
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Gene silencing vector pSilent-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:442632"
                     /note="Gene silencing vector for ascomysete fungi"
     CDS             129..989
                     /note="unnamed protein product; Ampicillin resistance
                     gene"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="BAF62257.1"
                     /db_xref="GI:146455413"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      2226..2592
                     /regulatory_class="promoter"
                     /note="Aspergillus nidulans TrpC gene"
     CDS             2593..3618
                     /note="unnamed protein product; Hygromycin resistance
                     gene"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="BAF62258.1"
                     /db_xref="GI:146455414"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE"
     regulatory      3638..4123
                     /regulatory_class="terminator"
                     /note="Aspergillus nidulans TrpC gene"
     regulatory      4162..5447
                     /regulatory_class="promoter"
                     /note="Aspergillus nidulans TrpC gene"
     misc_feature    5451..5476
                     /note="multi cloning site"
     intron          5491..5624
                     /note="Magnaporthe grisea cutinase gene"
     misc_feature    5637..5673
                     /note="multi cloning site"
     regulatory      5675..6404
                     /regulatory_class="terminator"
                     /note="Aspergillus nidulans TrpC gene"
ORIGIN     
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
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