pSK+KanaRpsL

$160.00

  • Model: PVT10537
  • 50 Units in Stock
Ask a question


Add to Cart:

pSK+KanaRpsL

Catalog No. PVT10537
Packing 2ug

 

pSK+KanaRpsL Information

Function Other source plasmids

Replicator: pUC ori, F1 ori

Plasmid classification: gene library plasmid; other gene plasmids; other source plasmids.

Plasmid size: 4678bp

Plasmid label: Kana-RpsL

Prokaryotic resistance: ampicillin Amp (100 u g/ml)

Cloned strains of Escherichia coli, DH5 A and other Escherichia coli

Culture conditions: 37 centigrade, aerobic, LB

Expression host: mammalian cells such as 293T

Culture conditions: 37 C, 5%CO2

Induction mode: no induction

 

pSK+KanaRpsL Description

PSK+KanaRpsL has a complete KanaRpsL gene template.

 

pSK+KanaRpsL Sequence

LOCUS       Exported                4678 bp ds-DNA     circular SYN 14-AUG-2017
DEFINITION  synthetic circular DNA
KEYWORDS    pSK+KanaRpsL.dna
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4678)
  TITLE     Direct Submission
  JOURNAL   Exported Aug 14, 2017  
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1031..1405
                     /codon_start=1
                     /label=RpsL
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKP
                     NSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGALDC
                     SGVKDRKQARSKYGVKRPKA"
     CDS             1446..2240
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2487..2505)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(2928..3516)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3687..4547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4548..4652)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttcacgct gccgcaagca ctcagggcgc
      721 aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
      781 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
      841 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
      901 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
      961 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctaaccag
     1021 gagctattta atggcaacag ttaaccagct ggtacgcaaa ccacgtgctc gcaaagttgc
     1081 gaaaagcaac gtgcctgcgc tggaagcatg cccgcaaaaa cgtggcgtat gtactcgtgt
     1141 atatactacc actcctaaaa aaccgaactc cgcgctgcgt aaagtatgcc gtgttcgtct
     1201 gactaacggt ttcgaagtga cttcctacat cggtggtgaa ggtcacaacc tgcaggagca
     1261 ctccgtgatc ctgatccgtg gcggtcgtgt taaagacctc ccgggtgttc gttaccacac
     1321 cgtacgtggt gcgcttgact gctccggcgt taaagaccgt aagcaggctc gttccaagta
     1381 tggcgtgaag cgtcctaagg cttaatggta gatctgatca agagacagga tgacggtcgt
     1441 ttcgcatgct tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     1501 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     1561 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     1621 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     1681 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     1741 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     1801 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     1861 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     1921 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     1981 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     2041 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     2101 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     2161 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     2221 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     2281 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     2341 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     2401 cttcgcccac cccgggctcg atcccctcgg ggggatccac tagttctaga gcggccgcca
     2461 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattccgag cttggcgtaa
     2521 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2581 cgagccggaa gcataaagtg taaa
No customer comments for the moment.

Add A Comment

Related Products

Cart  

No products

Total $0.00

Prices don't include postage.

Cart