pStat3- TA- luc

$193.00

  • Model: PVT10815
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pStat3-TA-luc

Catalog No. PVT10815
Packing 2ug

 

pStat3-TA-luc Information

Function Mammal reporter plasmid

Promoter: SV40

Replicator: pUC ori, SV40 ori

Terminator: SV40 poly (A) signal

Plasmid classification: mammalian cells, signal pathway reporting vectors

Plasmid size: 5672bp

Prokaryotic resistance: Amp

Cloned strain: DH5 alpha

Culture conditions: 37 centigrade, aerobic LB

Expression host: mammalian cells

Induction mode: no induction, instantaneous expression

5'sequencing primers: primers designed according to sequence

3'sequencing primers: primers designed according to sequence

 

pStat3-TA-luc Description

PSTAT3-TA-luc can be transfected into cells by conventional cell transfection method. The biyuntian firefly firefly firefly firefly luciferase reporter gene assay kit detection (RG005/RG006) or dual luciferase reporter gene assay kit (RG027/RG028). 

STAT3 reagent can be activated, can be used as a positive control pSTAT3-TA-luc report gene detection.

pSTAT3-TA-luc

 

pStat3-TA-luc Sequence

LOCUS       Exported                5672 bp ds-DNA     circular SYN 04-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, September 4, 2016 from SnapGene Viewer 3.1.4
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        109..140
                     /note="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             173..1825
                     /codon_start=1
                     /product="firefly luciferase"
                     /note="luciferase"
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1869..1990
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        2184..2541
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      2392..2527
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             2572..3366
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3391..3439
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3766..4354)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4554..5414)
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5519..5567
                     /note="synthetic polyadenylation signal"
     misc_feature    5581..5672
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccg ctagcctcga gtgcttcccg aacgttgctt cccgaacgtt
       61 gcttcccgaa cgttgcttcc gaacgtagat ctgcagaagc ttagacacta gagggtatat
      121 aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca ccatggaaga
      181 tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg
      241 cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac
      301 cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct
      361 ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga
      421 gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc
      481 cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc
      541 caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct
      601 accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag
      661 catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc
      721 cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg
      781 attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg
      841 cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt
      901 tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt
      961 gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca
     1021 atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa
     1081 gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt
     1141 aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac
     1201 agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg
     1261 caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg
     1321 tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa
     1381 caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat
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