pT2/ VENUS Plasmid

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  • Model: PVT1041
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pT2/ VENUS

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pT2/VENUS,Plasmid pT2/VENUS,pT2/VENUS vector

pT2/ VENUS plasmid  information

Promoters: T3, T7, chicken beta -actin
Replicon: pUC ori
Terminator: SV40 poly (A) signal
Plasmid classification: mammalian cells, protein overexpression vector
Plasmid size: 6123bp
Prokaryotic resistance: Amp
Clone strain: DH5 alpha
Culture conditions: 37 LB, aerobic
Expression host: mammalian cells
Induction method: no induction, transient expression
Primers for 5'sequencing: primers were designed according to the sequence
Primers for 3'sequencing: primers were designed according to the sequence

pT2/ VENUS plasmid Sequence

LOCUS       Exported                6123 bp ds-DNA     circular SYN 29-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pT2VENUS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, August 29, 2016 from SnapGene Viewer 3.1.4
            
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(61..79)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(100..116)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    124..140
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(148..178)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    193..214
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(502..1090)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1261..2121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2122..2226)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      2253..2708
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     2849..2865
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2875..2893
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3295..3416
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     intron          4169..5177
                     /note="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        complement(5178..5453)
                     /note="chicken beta-actin promoter"
     enhancer        5456..5759
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggatcctct agctagagtc gacctcgatc gagggggggc ccggtaccca gcttttgttc
       61 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
      121 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      181 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      241 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      301 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      361 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      421 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      481 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      541 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      601 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      661 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      721 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      781 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      841 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      901 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
      961 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1021 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1081 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1141 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1201 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1261 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1321 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1381 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1441 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1501 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1561 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1621 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1681 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1741 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1801 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1861 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     1921 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     1981 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2041 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2101 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2161 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2221 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
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