pUC-SPYNE(R)173
Catalog No. PVT11202
Packing 2ug
Function plant reporter plasmid
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon PUC
Copy Middle
Promoter 35s
Induction --
Forward primer 35s
Reverse primer
pUC-SPYNE(R)173 Information
Promoter: 35S
Terminator: NOS
Prokaryotic resistance: ampicillin Amp
Cloned strain: Escherichia coli DH5 alpha
Culture conditions: 37 degrees centigrade
pUC-SPYNE(R)173 Description
PUC-SPYNE (R) 173 is a plant cell double molecule fluorescent BIFC plasmid.
pUC-SPYNE(R)173 Sequence
LOCUS Exported 4380 bp ds-DNA circular SYN 04-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..4380
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 863..1208
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 1223..1741
/codon_start=1
/product="N-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VN173
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIE"
CDS 1742..1771
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
misc_feature 1772..1831
/label=MCS
terminator 1842..2094
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
promoter 2593..2697
/gene="bla"
/label=AmpR promoter
CDS 2698..3558
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3729..4317
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
61 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
241 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
301 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
361 tgcctgcagg tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg
421 ttagagaggc ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg
481 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca
541 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga
601 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag
661 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg
721 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa
781 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
841 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
901 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
961 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
1021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
1081 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
1141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
1201 agagaacacg ggggactcta gaatggtgag caagggcgag gagctgttca ccggggtggt
1261 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
1321 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
1381 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc
1441 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
1501 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
1561 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
1621 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
1681 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
1741 ggagcaaaag ttgatttctg aggaggatct tgggcccagg cctactagtg gatccgtcga
1801 cctcgagggt accgctcccg ggtaggagct cgaatttccc cgatcgttca aacatttggc
1861 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
1921 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
1981 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
2041 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
2101 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
2161 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
2221 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
2281 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
2341 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
2401 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
2461 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
2521 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
2581 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
2641 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
2701 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
2761 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
2821 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
2881 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
2941 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
3001 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
3061 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
3121 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
3181 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct