pUC- SPYNE (R)173

$215.00

  • Model: PVT11202
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pUC-SPYNE(R)173

Catalog No. PVT11202
Packing 2ug
Function plant reporter plasmid
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon PUC
Copy Middle
Promoter 35s
Induction --
Forward primer 35s
Reverse primer

 

pUC-SPYNE(R)173 Information

Promoter: 35S

Terminator: NOS

Prokaryotic resistance: ampicillin Amp

Cloned strain: Escherichia coli DH5 alpha

Culture conditions: 37 degrees centigrade

 

pUC-SPYNE(R)173  Description

PUC-SPYNE (R) 173 is a plant cell double molecule fluorescent BIFC plasmid.

pUC-SPYNE(R)173-vector

 

pUC-SPYNE(R)173  Sequence

LOCUS       Exported                4380 bp ds-DNA     circular SYN 04-JAN-2018
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        863..1208
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1223..1741
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     CDS             1742..1771
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    1772..1831
                     /label=MCS
     terminator      1842..2094
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2593..2697
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2698..3558
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3729..4317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
       61 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      241 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      301 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
      361 tgcctgcagg tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg
      421 ttagagaggc ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg
      481 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca
      541 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga
      601 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag
      661 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg
      721 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa
      781 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
      841 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
      901 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
      961 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
     1021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
     1081 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
     1141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
     1201 agagaacacg ggggactcta gaatggtgag caagggcgag gagctgttca ccggggtggt
     1261 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1321 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1381 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc
     1441 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1501 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1561 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1621 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1681 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1741 ggagcaaaag ttgatttctg aggaggatct tgggcccagg cctactagtg gatccgtcga
     1801 cctcgagggt accgctcccg ggtaggagct cgaatttccc cgatcgttca aacatttggc
     1861 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     1921 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     1981 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     2041 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
     2101 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2161 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     2221 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     2281 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     2341 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2401 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2461 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     2521 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2581 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2641 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2701 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2761 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2821 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2881 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2941 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3001 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3061 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3121 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3181 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
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