pUC19

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  • Model: PVT0003
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pUC19

PVT0003       2ug

 

pUC19 Information

Promoter: Lac/lac promoter

Replicon: ColE1 ori

Plasmid classification: Escherichia coli vector; cloned plasmid

Plasmid size: 2686bp

Plasmid label: LacZ

Prokaryotic resistance: ampicillin Amp

Cloned strain: DH5 alpha

Culture conditions: 37 centigrade, aerobic, LB

5'sequencing primers: M13F:TGTAAAACGACGGCCAGT

3'sequencing primers: M13R:CAGGAAACAGCTATGACC

Note: selection of blue leukoplakia

 

pUC19 Description

The size of pUC18 and pUC19 is only 2686bp, the most commonly used plasmid vector with compact structure, almost free of excess DNA, and GenBank registration number of L08752 (pUC18) and X02514 (pUC19). From pBR322, lacZ (MSC) comes from M13mp18/19. The structure of these two plasmids is almost the same, but the direction of the polyclonal sites is opposite. These plasmids lack ROP gene that controls copy number, so the copy number is 500-700. The pUC series contains a partial coding sequence of the amino terminal of a lacZ protein, which can exhibit alpha complementarity in specific receptor cells. Therefore, after inserting foreign fragments into polyclonal sites, the recombinant plasmids can be screened through blue and white colonies formed by alpha complementarity. PUC18 and pUC19 vectors are suitable for cloning DNA fragments, sequencing DNA, and expressing foreign genes. Because of the polyclonal loci in the lacZ field, it can be easily screened by blue and white to judge whether there is a DNA fragment insertion in the carrier when the lacZ deficient cell is transformed into a host (such as JM109 and so on) and is cultured on a flat medium containing IPTG and X-Gal. At the same time, foreign genes can be expressed by lac promoter on the vector, and DNA fragments in insertion vectors can be sequenced. DNA sequencing can facilitate the use of M13 universal primers.

 

pUC19 Multiple site

pUC19-multiple-site

 

pUC19 Sequence

LOCUS       Exported File           2686 bp ds-DNA    circular SYN 02-2-2015
KEYWORDS    pUC19
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2015-2-2 
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    106..127
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             216..539
                     /codon_start=1
                     /gene="lacZ?fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /note="lacZ-alpha"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    233..289
                     /gene="
                     "
                     /note="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(290..306)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        780..884
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             885..1745
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1916..2504
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
      301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
      541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
      601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
      661 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
      721 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2041 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2521 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2581 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2641 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaga
//

 
Caution:
Product is for research use only!
 

Search name

pUC19,Plasmid pUC19,pUC19 vector

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