pVE5523 Plasmid

$216.00

  • Model: PVT5203
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pVE5523

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pVE5523,Plasmid pVE5523,pVE5523 vector

pVE5523 plasmid information

Promoter: P59
Replicon: colE1 ori
Terminator: rrnB T1, rrnB T2
Plasmid classification: secretory expression plasmid of Lactobacilli
Plasmid size: 8328bp
Plasmid label: PSusp
Prokaryotic resistance: ampicillin Ampicillin
Screening markers: erythromycin Erythromycin
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: Lactobacilli
5'sequencing primers: Based on sequence design
3'sequencing primers: Based on sequence design
Remarks: secretory expression
Use:Lactic Acid Bacteria Plasmids

pVE5523 plasmid Sequence

 

LOCUS       Exported                8328 bp ds-DNA     circular SYN 15-JUN-2017
DEFINITION  synthetic circular DNA
KEYWORDS    pVE5523
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8328)
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, June 15, 2017 from SnapGene Viewer 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(2819..2835)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3309..3413
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3414..4274
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4445..5033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5321..5342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5357..5387
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5395..5411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5419..5435
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5456..5474
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      6358..6444
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6463..6490
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             7104..7841
                     /codon_start=1
                     /label=Ery
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
ORIGIN
        1 aactcaacgc tagtagtgga tttaatccca aatgagccaa cagaaccaga accagaaaca
       61 gaatcagaac aagtaacatt ggatttagaa atggaagaag aaaaaagcaa tgacttcgtg
      121 tgaataatgc acgaaatcgt tgcttatttt tttttaaaag cggtatacta gatataacga
      181 aacaacgaac tgaatagaaa cgaaaaaaga gccatgacac atttataaaa tgtttgacga
      241 cattttataa atgcatagcc cgataagatt gccaaaccaa cgcttatcag ttagtcagat
      301 gaactcttcc ctcgtaagaa gttatttaat taactttgtt tgaagacggt atataaccgt
      361 actatcatta tatagggaaa tcagagagtt ttcaagtatc taagctactg aatttaagaa
      421 ttgttaagca atcaatcgga aatcgtttga ttgctttttt tgtattcatt tatagaaggt
      481 ggagtttgta tgaatcatga tgaatgtaaa acttatataa aaaatagttt attggagata
      541 agaaaattag caaatatcta tacactagaa acgtttaaga aagagttaga aaagagaaat
      601 atctacttag aaacaaaatc agataagtat ttttcttcgg agggggaaga ttatatatat
      661 aagttaatag aaaataacaa aataatttat tcgattagtg gaaaaaaatt gacttataaa
      721 ggaaaaaaat ctttttcaaa acatgcaata ttgaaacagt tgaatgaaaa agcaaaccaa
      781 gttaattaaa caacctattt tataggattt ataggaaagg agaacagctg aatgaatatc
      841 ccttttgttg tagaaactgt gcttcatgac ggcttgttaa agtacaaatt taaaaatagt
      901 aaaattcgct caatcactac caagccaggt aaaagcaaag gggctatttt tgcgtatcgc
      961 tcaaaatcaa gcatgattgg cggtcgtggt gttgttctga cttccgagga agcgattcaa
     1021 gaaaatcaag atacatttac acattggaca cccaacgttt atcgttatgg aacgtatgca
     1081 gacgaaaacc gttcatacac gaaaggacat tctgaaaaca atttaagaca aatcaatacc
     1141 ttctttattg attttgatat tcacacggca aaagaaacta tttcagcaag cgatatttta
     1201 acaaccgcta ttgatttagg ttttatgcct actatgatta tcaaatctga taaaggttat
     1261 caagcatatt ttgttttaga aacgccagtc tatgtgactt caaaatcaga atttaaatct
     1321 gtcaaagcag ccaaaataat ttcgcaaaat atccgagaat attttggaaa gtctttgcca
     1381 gttgatctaa cgtgtaatca ttttggtatt gctcgcatac caagaacgga caatgtagaa
     1441 ttttttgatc ctaattaccg ttattctttc aaagaatggc aagattggtc tttcaaacaa
     1501 acagataata agggctttac tcgttcaagt ctaacggttt taagcggtac agaaggcaaa
     1561 aaacaagtag atgaaccctg gtttaatctc ttattgcacg aaacgaaatt ttcaggagaa
     1621 aagggtttaa tagggcgtaa taacgtcatg tttaccctct ctttagccta ctttagttca
     1681 ggctattcaa tcgaaacgtg cgaatataat atgtttgagt ttaataatcg attagatcaa
     1741 cccttagaag aaaaagaagt aatcaaaatt gttagaagtg cctattcaga aaactatcaa
     1801 ggggctaata gggaatacat taccattctt tgcaaagctt gggtatcaag tgatttaacc
     1861 agtaaagatt tatttgtccg tcaagggtgg tttaaattca agaaaaaaag aagcgaacgt
     1921 caacgtgttc atttgtcaga atggaaagaa gatttaatgg cttatattag cgaaaaaagc
     1981 gatgtataca agccttattt agtgacgacc aaaaaagaga ttagagaagt gctaggcatt
     2041 cctgaacgga cattagataa attgctgaag gtactgaagg cgaatcagga aattttcttt
     2101 aagattaaac caggaagaaa tggtggcatt caacttgcta gtgttaaatc attgttgcta
     2161 tcgatcatta aagtaaaaaa agaagaaaaa gaaagctata taaaggcgct gacaaattct
     2221 tttgacttag agcatacatt cattcaagag actttaaaca agctagcaga acgccctaaa
     2281 acggacacac aactcgattt gtttagctat gatacaggct gaaaataaaa cccgcactat
     2341 gccattacat ttatatctat gatacgtgtt tgttttttct ttgctgttta gcgaatgatt
     2401 agcagaaata tacagagtaa gattttaatt aattattagg gggagaagga gagagtagcc
     2461 cgaaaacttt tagttggctt ggactgaacg aagtgaggga aaggctacta aaacgtcgag
     2521 gggcagtgag agcgaagcga acacttgatt ttttaatttt ctatctttta taggtcatta
     
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