pVL1392 Plasmid

$216.00

  • Model: PVT5108
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pVL1392

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pVL1392,Plasmid pVL1392,pVL1392 vector

pVL1392 plasmid information

Promoter: Polyhedrin
Replicon: ColE1 ori
Terminator: polyhedrin 3
Plasmid classification: baculovirus constitutive expression plasmids in insect cells
Plasmid size: 9032bp
Prokaryotic resistance: ampicillin Ampicillin
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: insect cells
Induction mode: constituent expression without induction
5'sequencing primers: Polyhedrin-F:AAATGATAACCATCTCGC
3'sequencing primers: Polyhedrin-R:GTCCAAGTTTCCCTG
Use:Insect cell plasmid

pVL1392 plasmid description

pVL1392 and pVL1393 are 9.8 kb vectors designed for use as baculovirus transfer vectors. These transfer vectors contain recombination sequences which are homologous to sequences in the baculovirus genome.Upon cotransfection, these sequences undergo homologous recombination resulting in a recombinant viral genome packaged in infectious viral particles.

These vectors are nonfusion vectors, which provide the AcMNPV polyhedrin enhancer-promoter sequences to drive high expression, and a multiple cloning site, conveniently constructed in opposite orientations, for simple subcloning. Translation initiation sequences must be provided by the inserted cDNA.

• Multiple cloning site polylinker, positioned in opposite orientations

• Recombination sequences for insertion into the baculovirus genome

• Polyhedrin enhancer-promoter sequences for high expression of recombinant protein Features for growth and maintenance in E. coli: • Ampicillin gene for selection

• pMB1 origin of replication from pUC19 for high copy number growth

pVL1392

pVL1392 vector

pVL1392 plasmid Sequence
 

LOCUS       Exported                9632 bp ds-DNA     circular SYN 26-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pVL1392
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, August 26, 2016 from SnapGene Viewer 3.1.4
            
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_recomb     3170..3997
                     /note="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     CDS             3170..3294
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /note="lef2"
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(3332..3937)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /note="ORF603"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        4001..4092
                     /gene="polh from Autographa californica"
                     /note="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_recomb     4654..5359
                     /note="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     3'UTR           4742..5114
                     /note="polyhedrin 3' UTR"
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(4771..5359)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /note="ORF1629"
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(5793..5810)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     primer_bind     complement(7013..7029)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7037..7053
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7061..7091)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7106..7127
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7415..8003)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8174..9034)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9035..9139)
                     /gene="bla"
                     /note="AmpR promoter"
     primer_bind     9613..9629
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