pVSV-G
Search name
pVSV-G,Plasmid pVSV-G,pVSV-G vector
pVSV-G Information
Promoter: CMV, T7
Replicon: pUC ori, SV40 ori
Terminator: beta -globin poly (A) signal
Plasmid classification: virus series, retrovirus packaging carrier
Plasmid size: 6363bp
Plasmid tagging: VSV-G
Prokaryotic resistance: Amp
Clone strain: Stbl3
Culture conditions: 37 C, aerobic LB
Expression host: lactation cells
Induction mode: no need to induce, transient expression.
5'sequencing primers: CMV-F:CGCAAATGGGCGGTAGGCGTG
Primers for 3'sequencing: primers designed based on sequences
pVSV-G Description
PVSV-G expressed G protein vesicular stomatitis virus (VSV-G) under the control of CMV immediate early promoter. VSV-G is used for pseudo-Moloney Murine Leukenia virus (MMLV) - mediated virus entry through retroviral vector VSV-G and phospholipids. Target cell membrane components, and promote the fusion of virus and cell membrane. The use of unnecessary cell surface receptors can serve as an alternative to viral envelope proteins. The pVSV-G vector includes intravenous infusion to enhance the stability of the synthesized intron mRNA, replication and ampicillin resistance to the spread of the bacterial mol E1 origin (Ampr) gene and the selection of antibiotics in the bacteria.
pVSV-G Multiple cloning site
pVSV-G Sequence
LOCUS Exported 6363 bp ds-DNA circular SYN 06-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 12
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6363)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, September 6, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..6363
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 83..462
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 463..666
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 778..1350
/note="beta-globin intron"
/note="intron from rabbit beta-globin gene"
CDS 1420..2955
/codon_start=1
/product="vesicular stomatitis virus G glycoprotein"
/note="VSV-G"
/note="Indiana strain"
/translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
polyA_site 3119..3174
/note="beta-globin poly(A) signal"
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(3532..3548)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3556..3572
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3580..3610)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 3625..3646
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3934..4522)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4693..5553)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5554..5658)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(5684..6139)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 6281..6297
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 6307..6325
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
61 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
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