pWB980

PVT5015    2ug

 

pWB980 Information

Bacterial Resistance:Kanamycin

Promoter: P43

Plasmid classification: the wide host series, Bacillus subtilis carrier

Plasmid size: 3787bp

Prokaryotic resistance: kanamycin Kan

Bacillus subtilis: Bacillus subtilis

Culture conditions: 37 centigrade, aerobic, LB

Expression host: Bacillus subtilis

5'sequencing primers: primers designed according to sequence

 

pWB980 Description

pWB705 andpWB980 . pUB110 is the parental plasmid for pUB18 and its derivatives.pWB705 is a pUB18-based secretion vector carrying both the constitutive expressed P43 promoter and the engineered levansucrase signal sequence. pWB980 is a pUB19-based secretion vector. The transcription direction of the P43 promoter is the same as that for the majority of the pUB110-encoded genes. rep, kan, ble, mob and sacB SP represent thesequence coding for replicase, kanamycin resistance marker,bleomycin resistance marker, mobilization protein and the levansucrase signal sequence, respectively. The arrows show the transcription directions for these genes.

 

pWB980 Reference

Article: Cloning and Expression of Staphylococcus Simulans Lysostaphin Enzyme Gene in Bacillus Subtilis WB600

Far BE, Ragheb M, Rahbar R, Mafakher L, Nojookambari NY, Achinas S, Yazdansetad S
Preprint from Research Square, 16 Dec 2020
DOI: 10.21203/rs.3.rs-122682/v1 
PPR: PPR254002 

 

pWB980 Sequence