pWB980
PVT5015 2ug
pWB980 Information
Bacterial Resistance:Kanamycin
Promoter: P43
Plasmid classification: the wide host series, Bacillus subtilis carrier
Plasmid size: 3787bp
Prokaryotic resistance: kanamycin Kan
Bacillus subtilis: Bacillus subtilis
Culture conditions: 37 centigrade, aerobic, LB
Expression host: Bacillus subtilis
5'sequencing primers: primers designed according to sequence
pWB980 Description
pWB705 andpWB980 . pUB110 is the parental plasmid for pUB18 and its derivatives.pWB705 is a pUB18-based secretion vector carrying both the constitutive expressed P43 promoter and the engineered levansucrase signal sequence. pWB980 is a pUB19-based secretion vector. The transcription direction of the P43 promoter is the same as that for the majority of the pUB110-encoded genes. rep, kan, ble, mob and sacB SP represent thesequence coding for replicase, kanamycin resistance marker,bleomycin resistance marker, mobilization protein and the levansucrase signal sequence, respectively. The arrows show the transcription directions for these genes.
pWB980 Reference
Article: Cloning and Expression of Staphylococcus Simulans Lysostaphin Enzyme Gene in Bacillus Subtilis WB600
Far BE, Ragheb M, Rahbar R, Mafakher L, Nojookambari NY, Achinas S, Yazdansetad S
Preprint from Research Square, 16 Dec 2020
DOI: 10.21203/rs.3.rs-122682/v1
PPR: PPR254002
pWB980 Sequence
LOCUS Exported 3787 bp ds-DNA circular SYN 29-AUG-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3787)
TITLE Direct Submission
JOURNAL Exported Aug 29, 2017
FEATURES Location/Qualifiers
source 1..3787
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 309..1313
/codon_start=1
/gene="repB"
/product="RepB replication protein"
/label=repB
/note="from Enterococcus faecalis plasmid pAM-alpha-1"
/db_xref="GI:22652809"
/protein_id="AAN03827.1"
/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
ORIGIN
1 agaacctaaa aagaacgaat ttgaactaac tcataaccga gaggtaaaaa aagaacgaag
61 tcgagatcag ggaatgagtt tataaaataa aaaaagcacc tgaaaaggtg tctttttttg
121 atggttttga acttgttctt tcttatcttg atacatatag aaataacgtc atttttattt
181 tagttgctga aaggtgcgtt gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc
241 ttaaaattgg ttgcacagaa aaaccccatc tgttaaagtt ataagtgact aaacaaataa
301 ctaaatagat gggggtttct tttaatatta tgtgtcctaa tagtagcatt tattcagatg
361 aaaaatcaag ggttttagtg gacaagacaa aaagtggaaa agtgagacca tggagagaaa
421 agaaaatcgc taatgttgat tactttgaac ttctgcatat tcttgaattt aaaaaggctg
481 aaagagtaaa agattgtgct gaaatattag agtataaaca aaatcgtgaa acaggcgaaa
541 gaaagttgta tcgagtgtgg ttttgtaaat ccaggctttg tccaatgtgc aactggagga
601 gagcaatgaa acatggcatt cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc
661 caacagttcg ttggttgttt ctcacattaa cagttaaaaa tgtttatgat ggcgaagaat
721 taaataagag tttgtcagat atggctcaag gatttcgccg aatgatgcaa tataaaaaaa
781 ttaataaaaa tcttgttggt tttatgcgtg caacggaagt gacaataaat aataaagata
841 attcttataa tcagcacatg catgtattgg tatgtgtgga accaacttat tttaagaata
901 cagaaaacta cgtgaatcaa aaacaatgga ttcaattttg gaaaaaggca atgaaattag
961 actatgatcc aaatgtaaaa gttcaaatga ttcgaccgaa aaataaatat aaatcggata
1021 tacaatcggc aattgacgaa actgcaaaat atcctgtaaa ggatacggat tttatgaccg
1081 atgatgaaga aaagaatttg aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa
1141 ggttaatctc ctatggtggt ttgttaaaag aaatacataa aaaattaaac cttgatgaca
1201 cagaagaagg cgatttgatt catacagatg atgacgaaaa agccgatgaa gatggatttt
1261 ctattattgc aatgtggaat tgggaacgga aaaattattt tattaaagag tagttcaaca
1321 aacgggccag tttgttgaag attagatgct ataattgtta ttaaaaggat tgaaggatgc
1381 ttaggaagac gagttattaa tagctgaata agaacggtgc tctccaaata ttcttattta
1441 gaaaagcaaa tctaaaatta tctgaaaagg gaatgagaat agtgaatgga ccaataataa
1501 tgactagaga agaaagaatg aagattgttc atgaaattaa ggaacgaata ttggataaat
1561 atggggatga tgttaaggct attggtgttt atggctctct tggtcgtcag actgatgggc
1621 cctattcgga tattgagatg atgtgtgtca tgtcaacaga ggaagcagag ttcagccatg
1681 aatggacaac cggtgagtgg aaggtggaag tgaattttga tagcgaagag attctactag
1741 attatgcatc tcaggtggaa tcagattggc cgcttacaca tggtcaattt ttctctattt
1801 tgccgattta tgattcaggt ggatacttag agaaagtgta tcaaactgct aaatcggtag
1861 aagcccaaac gttccacgat gcgatttgtg cccttatcgt agaagagctg tttgaatatg
1921 caggcaaatg gcgtaatatt cgtgtgcaag gaccgacaac atttctacca tccttgactg
1981 tacaggtagc aatggcaggt gccatgttga ttggtctgca tcatcgcatc tgttatacga
2041 cgagcgcttc ggtcttaact gaagcagtta agcaatcaga tcttccttca ggttatgacc
2101 atctgtgcca gttcgtaatg tctggtcaac tttccgactc tgagaaactt ctggaatcgc
2161 tagagaattt ctggaatggg attcaggagt ggacagaacg acacggatat atagtggatg
2221 tgtcaaaacg cataccattt tgaacgatga cctctaataa ttgttaatca tgttggttac
2281 gtatttatta acttctccta gtattagtaa ttatcatggc tgtcatggcg cattaacgga
2341 ataaagggtg tgcttaaatc gggccatttt gcgtaataag aaaaaggatt aattatgagc
2401 gaattgaatt aataataagg taatagattt acattagaaa atgaaagggg attttatgcg
2461 tgagaatgtt acagtctatc ccggcattgc cagtcgggga tattaaaaag agtataggtt
2521 tttattgcga taaactaggt ttcactttgg ttcaccatga agatggattc gcagttctaa
2581 tgtgtaatga ggttcggatt catctatggg aggcaagtga tgaaggctgg cgctctcgta
2641 gtaatgattc accggtttgt acaggtgcgg agtcgtttat tgctggtact gctagttgcc
2701 gcattgaagt agagggaatt gatgaattat atcaacatat taagcctttg ggcattttgc
2761 accccaatac atcattaaaa gatcagtggt gggatgaacg agactttgca gtaattgatc
2821 ccgacaacaa tttgattagc ttttttcaac aaataaaaag ctaaaatcta ttattaatct
2881 gttcagcaat cgggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt
2941 ttattttgag tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc
3001 ttgctgagtc tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt
3061 ttaaaggtgg agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt
3121 tttaaacgag cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt
3181 gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac
3241 tgaattcgag ctcagcatta ttgagtggat gattatattc cttttgatag gtggtatgtt
3301 ttcgcttgaa cttttaaata cagccattga acatacggtt gatttaataa ctgacaaaca
3361 tcaccctctt gctaaagcgg ccaaggacgc tgccgccggg gctgtttgcg tttttgccgt
3421 gatttcgtgt atcattggtt tacttatttt tttgccaaag ctgtaatggc tgaaaattct
3481 tacatttatt ttacattttt agaaatgggc gtgaaaaaaa gcgcgcgatt atgtaaaata
3541 taaagtgata gcggtaccag gagggctgga agaagcagac cgctaacaca gtacataaaa
3601 aaggagacat gaacgatgaa catcaaaaag tttgcaaaac aagcaacagt attaaccttt
3661 actaccgcac tgctggcagg aggcgcaact caagcttttg cctcgagctc ggtacccggg
3721 gatcctctag agtcgacctg caggcatgca agctagcttc agcacaattc caagaaagac
3781 acgattt
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Product is for research use only!