pY26TEF- GPD

$260.00

  • Model: PVT11249
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pY26TEF-GPD

Catalog No. PVT11249   Packing 2ug


pY26TEF-GPD Description
PY26TEF-GPD is a high-copy bidirectional expression plasmid of Saccharomyces cerevisiae. TEF1 promoter and GAP promoter drive the expression of two genes respectively.


pY26TEF-GPD Information

Function yeast expression plasmid
Promoter: TEF1 promoter, GAP promoter
Replicon: 2 ori, pBR322 ori, F1 ori
Terminator: ADH1 terminator, CYC1 Terminator
Plasmid size: 7430bp
Prokaryotic resistance: ampicillin Amp
Selection marker: URA3
Clone strain: Escherichia coli DH5 alpha
Culture conditions: 37 C, aerobic, LB
Expression host: Saccharomyces cerevisiae

 

pY26TEF-GPD Multiple cloning site

https://lifescience-market.com/images/pY26TEF-GPD-vector.png

pY26TEF-GPD Sequence

LOCUS       Exported                7430 bp ds-DNA     circular SYN 05-NOV-2017
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7430)
            
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    401..448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1438..1542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1543..2403
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      3643..3807
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    3915..3982
                     /label=MCS1
     promoter        complement(3998..4396)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        4452..5095
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase"
     misc_feature    5107..5169
                     /label=MCS2
     terminator      5170..5417
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      5625..6080
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(6211..7014)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7015..7230)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct cgctattacg ccagctgaat tggagcgacc tcatgctata
     3661 ctgagaaagc aacctgacct acaggaaaga gttactcaag aataagaatt ttcgttttaa
     3721 aacctaagag tcactttaaa atttgtatac acttattttt tttataactt atttaataat
     3781 aaaaatcata aatcataaga aattcgctta tttagaagtg tcaacaacgt atctaccaac
     3841 gatttgaccc ttttccatct tttcgtaaat ttctggcaag gtagacaagc cgacaacctt
     3901 gattggagac ttgaccaaac ctctggcgaa gaattgttaa ttaaagatct ccgcggtccg
     3961 gagttaactg atcagcggcc gcgctagttc tagaaaactt agattagatt gctatgcttt
     4021 ctttctaatg agcaagaagt aaaaaaagtt gtaatagaac aagaaaaatg aaactgaaac
     4081 ttgagaaatt gaagaccgtt tattaactta aatatcaatg ggaggtcatc gaaagagaaa
     4141 aaaatcaaaa aaaaaatttt caagaaaaag aaacgtgata aaaattttta ttgccttttt
     4201 cgacgaagaa aaagaaacga ggcggtctct tttttctttt ccaaaccttt agtacgggta
     4261 attaacgaca ccctagagga agaaagaggg gaaatttagt atgctgtgct tgggtgtttt
     4321 gaagtggtac ggcgatgcgc ggagtccgag aaaatctgga agagtaaaaa aggagtagaa
     4381 acattttgaa gctatgagct ccagcttttg ttccctttag tgagggttaa ttgcgcgccg
     4441 agctcagttt atcattatca atactcgcca tttcaaagaa tacgtaaata attaatagta
     4501 gtgattttcc taactttatt tagtcaaaaa attagccttt taattctgct gtaacccgta
     4561 catgcccaaa atagggggcg ggttacacag aatatataac atcgtaggtg tctgggtgaa
     4621 cagtttattc ctggcatcca ctaaatataa tggagcccgc tttttaagct ggcatccaga
     4681 aaaaaaaaga atcccagcac caaaatattg ttttcttcac caaccatcag ttcataggtc
     4741 cattctctta gcgcaactac agagaacagg ggcacaaaca ggcaaaaaac gggcacaacc
     4801 tcaatggagt gatgcaacct gcctggagta aatgatgaca caaggcaatt gacccacgca
     4861 tgtatctatc tcattttctt acaccttcta ttaccttctg ctctctctga tttggaaaaa
     4921 gctgaaaaaa aaggttgaaa ccagttccct gaaattattc ccctacttga ctaataagta
     4981 tataaagacg gtaggtattg attgtaattc tgtaaatcta tttcttaaac ttcttaaatt
     5041 ctacttttat agttagtctt ttttttagtt ttaaaacacc agaacttagt ttcgacggat
     5101 tctagaacta gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc
     5161 gacctcgagt catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg
     5221 ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta
     5281 tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca
     5341 gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct
     5401 cgaaggcttt aatttgcggc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg
     5461 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5521 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5581 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg
     5641 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5701 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5761 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5821 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5881 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5941 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6001 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6061 aatattaacg tttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     6121 tcacaccgca tagggtaata actgatataa ttaaattgaa gctctaattt gtgagtttag
     6181 tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc ttctcaaata
     6241 tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc ttccctttgc
     6301 aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat catccacggt
     6361 tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg gtgtcataat
     6421 caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa agccgataac
     6481 aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc cagtagatag
     6541 ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct ttgttacttc
     6601 ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt gtgcattcgt
     6661 aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt tgactgtatt
     6721 accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc
     6781 ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca catgtgtttt
     6841 tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct tggtggtacg
     6901 aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa atagcttggc
     6961 agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg acatgattta
     7021 tcttcgtttc ctgcaggttt ttgttctgtg cagttgggtt aagaatactg ggcaatttca
     7081 tgtttcttca acactacata tgcgtatata taccaatcta agtctgtgct ccttccttcg
     7141 ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaagaaacc gaaatcaaaa
     7201 aaaagaataa aaaaaaaatg atgaattgaa ttgaaaagct gtggtatggt gcactctcag
     7261 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     7321 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     7381 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
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