Plasmid classification: yeast vector, Saccharomyces cerevisiae expression plasmid
The pYX212 plasmid can be expressed in Saccharomyces cerevisiae, and the TPI promoter drives the expression of the target gene. The HA tag is on the C-terminal. WB test can be used to detect the expression of the gene conveniently.
LOCUS Exported 8346 bp ds-DNA circular SYN 05-JUN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pYX212
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8346)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8346
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..48
/note="MCS"
CDS 49..75
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
rep_origin 394..849
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(980..1783)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/note="URA3"
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVLGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVQGERYRKAGWEAYLRRCGQQN"
promoter complement(1784..1999)
/gene="S. cerevisiae URA3"
/note="URA3 promoter"
rep_origin 2853..4195
/note="2u ori"
/note="yeast 2u plasmid origin of replication"
promoter 4484..4588
/gene="bla"
/note="AmpR promoter"
CDS 4589..5449
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLARRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLDNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5620..6208
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 7436..8345
/note="TPI promoter"
ORIGIN
1 gaattcacca tggatcctag ggcccacaag cttacgcgtc gacccgggta tccgtatgat
61 gtgcctgact acgcatgata tctcgagctc agctagctaa ctgaataagg aacaatgaac
121 gtttttcctt tctcttgttc ctagtattaa tgactgaccg atacatccct tttttttttt
181 gtctttgtct agctccagct tttgttccct ttagtgaggg ttaattcaat tcactggccg
241 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
301 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
361 aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg
421 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
481 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
541 taaatcgggg gctcccttta gggttccgat ttagtggttt acggcacctc gaccccaaaa
601 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
661 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
721 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
781 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
841 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
901 agggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat
961 ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc
1021 ctgcttttct gtaacgttca ccctgtacct tagcatccct tccctttgca aatagtcctc
1081 ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt
1141 gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt
1201 aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt
1261 cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc
1321 atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct
1381 gcttcaaacc gctaacaata cctggcccca gcacaccgtg tgcattcgta atgtctgccc
1441 attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag
1501 caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct
1561 taactgtgcc ctccatcgaa aaatcagtca atatatccac atgtgttttt agtaaacaaa
1621 ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg
1681 aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac
1741 taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc
1801 tgcaggtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa
1861 cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct
1921 gttcggagat taccgaatca aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa
1981 aaaaaaatga tgaattgaat tgaaaagctg tggtatggtg cactctcagt acaatctgct
2041 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
2101 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
2161 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
2221 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtgc ggccgctcta
2281 gaactagtgg atcaattcca cggactatag actatactag tatactccgt ctactgtacg
2341 atacacttcc gctcaggtcc ttgtccttta acgaggcctt accactcttt tgttactcta
2401 ttgatccagc tcagcaaagg cagtgtgatc taagattcta tcttcgcgat gtagtaaaac
2461 tagctagacc gagaaagaga ctagaaatgc aaaaggcact tctacaatgg ctgccatcat
2521 tattatccga tgtgacgctg cagcttctca atgatattcg aatacgcttt gaggagatac
2581 agcctaatat ccgacaaact gttttacaga tttacgatcg tacttgttac ccatcattga
2641 attttgaaca tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat
2701 aataatatat agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa
2761 ctattgcatc tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt
2821 tccatcttgc acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag
2881 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
2941 cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt
3001 tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
3061 ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc
3121 ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat
3181 tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta
3241 ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc
3301 ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga
3361 taaaggcatc cccgattata ttctataccg atgtggattc cgcatacttt gtgaacagaa
3421 agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg
3481 tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat
3541 gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa
3601 tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata
3661 gggatatagc acagagatat atagcaaaga gatacttttc agcaatgttt gtggaagcgg
3721 tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc
3781 gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat
3841 aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa
3901 cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt
3961 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata
4021 tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc
4081 atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact
4141 cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatatg
4201 catagtaccg agaaactagt gcgaagtagt gatcaggtat tgctgttatc tgatgagtat
4261 acgttgtcct ggccacggca gaagcaccct tatcgctcca atttcccaca acattagtca
4321 actccgttag gcccttcatt gaaagaaatg aggtcatcaa atgtcttcca atgtgagatt
4381 ttgggccatt ttttatagca aagattgaat aaggcgcatt tttcttcaaa gctgcggccg
4441 cactctcact agtacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
4501 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cgtgataaat
4561 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
4621 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
4681 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
4741 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
4801 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcgctcg
4861 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
4921 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
4981 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgga
5041 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
5101 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
5161 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
5221 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
5281 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
5341 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
5401 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
5461 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
5521 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
5581 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
5641 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
5701 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
5761 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
5821 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
5881 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
5941 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
6001 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
6061 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
6121 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
6181 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
6241 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
6301 taaccgtatt accgcctttg agtgagctga taccggtcgc cgcagccgaa cgaccgagcg
6361 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
6421 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
6481 tgagcgcaac gcaattaatg tgagttacct cactcattag gcaccccagg ctttacactt
6541 tatgcttccg gctcctatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
6601 cagctatgac catgattacg ccaagctcga aatacgactc actatagggc gaattgggta
6661 ccgggccggc cgtcgagctt gatggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
6721 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgaaaaa
6781 aagcggttag ctcttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
6841 actcatggtt atggcaggaa ctgcataatt ctcttactgt catgccatcc gtaagatgct
6901 tttctgtgac tggtgtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
6961 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
7021 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
7081 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
7141 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
7201 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
7261 ggttattgtc tcatgagcga tacatatttg aatgtattta gaaaaataaa caaatagggg
7321 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
7381 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttggggatct
7441 acgtatggtc attcttcttc agattccctc atggagaagt gcggcagatg tatatgacag
7501 agtcgccagt ttccaagaga ctttattcag gcacttccat gataggcaag agagaagacc
7561 cagagatgtt gttgtcctag ttacacatgg tatttattcc agagtattcc tgatgaaatg
7621 gtttagatgg acatacgaag agtttgaatc gtttaccaat gttcctaacg ggagcgtaat
7681 ggtgatggaa ctggacgaat ccatcaatag atacgtcctg aggaccgtgc tacccaaatg
7741 gactgattgt gagggagacc taactacata gtgtttaaag attacggata tttaacttac
7801 ttagaataat gccatttttt tgagttataa taatcctacg ttagtgtgag cgggatttaa
7861 actgtgagga cctcaataca ttcagacact tctgacggta tcaccctact tattcccttc
7921 gagattatat ctaggaaccc atcaggttgg tggaagatta cccgttctaa gacttttcag
7981 cttcctctat tgatgttaca ctcggacacc ccttttctgg catccagttt ttaatcttca
8041 gtggcatgtg agattctccg aaattaatta aagcaatcac acaattctct cggataccac
8101 ctcggttgaa actgacaggt ggtttgttac gcatgctaat gcaaaggagc ctatatacct
8161 ttggctcggc tgctgtaaca gggaatataa agggcagcat aatttaggag tttagtgaac
8221 ttgcaacatt tactattttc ccttcttacg taaatatttt tctttttaat tctaaatcaa
8281 tctttttcaa ttttttgttt gtattctttt cttgcttaaa tctataacta caaaaaacac
8341 atacag
//
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