T7opt in pCAGGS (T7 RNA polymerase)

  • Model: PVT10506
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T7opt in pCAGGS(T7 RNA polymerase)


Catalog No. PVT10506
Packing 2ug
Function Protease plasmids
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon pUC
Copy high
Promoter CAG
Induction --
Forward primer pCAGGS-F
Reverse primer

 

T7opt in pCAGGS(T7 RNA polymerase) information

Promoter: CAG

Replicon: pUC

Prokaryotic resistance: ampicillin Amp

Cloned strain: Escherichia coli DH5 alpha

Culture conditions: 37 degrees centigrade

 

T7opt in pCAGGS(T7 RNA polymerase) Description

T7opt in pCAGGS (T7 RNA polymerase) is a T7 RNA polymerase expression plasmid.

 

T7opt in pCAGGS(T7 RNA polymerase) Sequence

LOCUS       Exported                7381 bp ds-DNA     circular SYN 26-DEC-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1725..4376
                     /codon_start=1
                     /label=T7 RNA polymerase
                     /translation="MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYE
                     MGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQ
                     FLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFK
                     KNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIE
                     STGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGI
                     TGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANV
                     ITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSRRISLE
                     FMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYY
                     WLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFE
                     YAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAK
                     KVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVM
                     TLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVE
                     AMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLG
                     QFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHD
                     SFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLN
                     LRDILESDFAFA"
     polyA_site      4450..4505
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        5039..5234
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5085..5220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5240..5374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5613..6201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6372..7232)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7233..7337)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7368..1664
                     /label=CAG promoter
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
      661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1681 tggttattgt gctgtctcat cattttggca aagaattcgc caccatgaac accatcaata
     1741 ttgccaagaa cgacttttct gatatcgagc tggccgctat tccattcaat acactggctg
     1801 accactacgg agagcggctg gcccgcgaac agctggctct ggagcatgaa agctatgaga
     1861 tgggagaagc ccgattcagg aagatgtttg agaggcagct gaaagctggg gaagtggcag
     1921 acaacgcagc cgctaagcca ctgattacca cactgctgcc caaaatgatc gccagaatta
     1981 atgattggtt cgaggaagtg aaggcaaaac gaggaaagag gcctaccgcc ttccagtttc
     2041 tgcaggagat caagccagaa gctgtggcat acatcaccat caagactacc ctggcatgcc
     2101 tgacaagcgc cgacaacaca actgtgcagg cagtcgcttc cgcaatcgga cgagctattg
     2161 aggacgaagc acgctttggg agaatccggg atctggaggc caagcacttc aagaaaaacg
     2221 tggaggaaca gctgaacaag agggtggggc atgtctataa gaaggccttc atgcaggtgg
     2281 tcgaggccga catgctgtca aagggactgc tgggaggaga ggcctggagc tcctggcaca
     2341 aagaagatag catccatgtg ggagtccgct gcatcgagat gctgattgaa tctactggga
     2401 tggtgagtct gcaccgacag aacgccggcg tggtcggaca ggactctgag acaatcgaac
     2461 tggctcccga gtatgccgaa gctattgcaa ctagagccgg agctctggca gggatcagtc
     2521 caatgttcca gccctgcgtg gtccccccta agccttggac tggcatcacc gggggcggat
     2581 actgggctaa tggaaggaga ccactggcac tggtgcgaac acactctaag aaagccctga
     2641 tgagatacga ggatgtctat atgcccgaag tgtataaggc catcaacatt gctcagaata
     2701 cagcatggaa aattaacaag aaagtgctgg ccgtcgctaa tgtgatcact aagtggaaac
     2761 attgtcccgt ggaggacatc cctgccattg aacgggagga actgcctatg aagccagagg
     2821 acatcgatat gaaccctgaa gctctgaccg catggaagcg cgcagccgct gcagtgtaca
     2881 gaaaggataa agcccggaag tcccggcgca tttctctgga gttcatgctg gaacaggcca
     2941 acaagtttgc taatcacaaa gcaatctggt tcccctacaa catggactgg cgcggacgag
     3001 tctatgccgt gagcatgttc aaccctcagg ggaatgatat gacaaagggc ctgctgactc
     3061 tggctaaggg gaaaccaatt gggaaggagg gctactattg gctgaaaatc cacggggcca
     3121 attgcgctgg cgtcgacaag gtgccattcc ccgagaggat caagttcatc gaggaaaacc
     3181 atgaaaatat tatggcatgt gccaagtctc ccctggagaa cacatggtgg gccgaacagg
     3241 atagtccttt ctgctttctg gccttctgtt ttgagtacgc tggagtgcag caccatgggc
     3301 tgagttataa ttgctccctg ccactggcct ttgacggctc ttgtagtgga atccagcact
     3361 tctccgcaat gctgagggat gaggtcggag gaagagcagt gaacctgctg ccatctgaga
     3421 cagtgcagga catctacggc attgtcgcca agaaagtgaa tgagatcctg caggctgacg
     3481 caattaacgg gact
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