YEplac181
Catalog No. PVT11241
Packing 2ug
YEplac181 Information
Function yeast expression plasmid
Resistance Amp
Screen LEU2
Strain DH5alpha
Culture temperature 37degrees centigrade
YEplac181 Multiple cloning site
YEplac181 Sequence
LOCUS Exported 5741 bp ds-DNA circular SYN 21-DEC-2017
DEFINITION Yeast episomal plasmid with a LEU2 marker.
COMMENT The sequence of this plasmid was reconstructed using the description
from Gietz and Sugino.
FEATURES Location/Qualifiers
source 1..5741
/organism="synthetic DNA construct"
/lab_host="Saccharomyces cerevisiae"
/mol_type="other DNA"
promoter 142..172
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 180..196
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 216..500
/codon_start=1
/gene="lacZ"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRI"
misc_feature 233..289
/label=MCS
/note="pUC19 multiple cloning site"
rep_origin 745..2091
/label=2u ori
/note="yeast 2u plasmid origin of replication"
CDS complement(2227..3321)
/codon_start=1
/gene="S. cerevisiae LEU2"
/product="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/label=LEU2
/note="yeast auxotrophic marker"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
promoter complement(3322..3729)
/gene="S. cerevisiae LEU2"
/label=LEU2 promoter
promoter 3835..3939
/gene="bla"
/label=AmpR promoter
CDS 3940..4800
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4971..5559
/direction=RIGHT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tatcggatcg tacttgttac ccatcattga attttgaaca
541 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
601 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
661 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
721 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
781 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
841 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
901 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
961 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
1021 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
1081 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
1141 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
1201 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
1261 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
1321 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
1381 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
1441 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
1501 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
1561 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
1621 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
1681 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
1741 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
1801 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
1861 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
1921 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
1981 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
2041 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat
English