Anti-ATG3 antibody

Product Name Anti-ATG3 antibody

Catalog No PAab00673

Packing 100ug

Form liquid

Alternative Name APG3 antibody, APG3L

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12- ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Immunogen ATG3 autophagy related 3 homolog (S. cerevisiae)

Specificity Human,Mouse ,Rat

Tested Application ELISA,WB,IHC

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human kidney slide using ATG3 Antibody at dilution of 1:50

WB use

mouse kidney tissue were subjected to SDS PAGE followed by western blot with PAab00673(ATG3 antibody) at dilution of 1:300

Protein Information

Gene ID64422

Uniprot ID Q9NT62

Calculated MW

location