Anti-DNA2 antibody

$275.00

  • Model: PAab02433
  • 50 Units in Stock
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Anti-DNA2 antibody

 

Product Name Anti-DNA2 antibody

Catalog No PAab02433

Packing 100ug

Form liquid

Alternative Name DNA2L antibody, KIAA0083

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double- strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)- dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

Immunogen DNA replication helicase 2 homolog (yeast)

Specificity Human, Mouse

Tested Application ELISA,IHC,IF,WB

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human cervical cancer usingDNA2 antibody at dilution of 1:50

WB use

mouse thymus tissue were subjected to SDS PAGE followed by western blot with PAab02433(DNA2 antibody) at dilution of 1:500

Protein Information

Gene ID1763

Uniprot ID P51530

Calculated MW

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