Anti-DNMT1 antibody

$300.00

  • Model: PAab02482
  • 50 Units in Stock
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Anti-DNMT1 antibody

 

Product Name Anti-DNMT1 antibody

Catalog No PAab02482

Packing 100ug

Form liquid

Alternative Name AIM antibody, CXXC9 antibody, DNMT

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).

Immunogen DNA (cytosine-5-)-methyltransferase 1

Specificity Human

Tested Application ELISA,IP,WB,IF

Recommended dilution WB: 1:500-1:2000; IP: 1:500-1:2000; IF: 1:10-1:100

IF use

Immunofluorescent analysis of HepG2 cells using anti-DNMT1 antibody at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

WB use

293T cells were subjected to SDS PAGE followed by western blot with anti-DNMT1 antibody at dilution of 1:1000

Protein Information

Gene ID1786

Uniprot ID P26358

Calculated MW 180-200 kDa

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