Anti-EIF3E antibody

Product Name Anti-EIF3E antibody

Catalog No PAab02706

Packing 100ug

Form liquid

Alternative Name EIF3S6 antibody,

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense- mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

Immunogen eukaryotic translation initiation factor 3, subunit E

Specificity Human, Mouse ,Rat

Tested Application ELISA,WB,IHC,IF

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human pancreas cancer usingEIF3E antibody at dilution of 1:100

WB use

rat lymph tissue were subjected to SDS PAGE followed by western blot with PAab02706(EIF3E antibody) at dilution of 1:100

Protein Information

Gene ID3646

Uniprot ID P60228

Calculated MW

location