Anti-EIF3J antibody

Product Name Anti-EIF3J antibody

Catalog No PAab02711

Packing 100ug

Form liquid

Alternative Name EIF3S1

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.

Immunogen eukaryotic translation initiation factor 3, subunit J

Specificity Human,Mouse ,Rat

Tested Application ELISA,WB,IHC,IF,IP

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human breast cancer usingEIF3J antibody at dilution of 1:100

WB use

HeLa cells were subjected to SDS PAGE followed by western blot with PAab02711(EIF3J antibody) at dilution of 1:500

Protein Information

Gene ID8669

Uniprot ID O75822

Calculated MW

location