Anti-GAPDH antibody

Product Name Anti-GAPDH antibody

Catalog No PAab03342

Packing 100ug

Form liquid

Alternative Name GAPD

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Immunogen glyceraldehyde-3-phosphate dehydrogenase

Specificity Human,Mouse ,Rat,Pig

Tested Application ELISA,IHC,WB,IP,IF,FC

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human breast cancer usingGAPDH antibody at dilution of 1:100

WB use

Western blot of Hela cell with anti-GAPDH (PAab03342) at various dilutions. PAab03342

Protein Information

Gene ID2597

Uniprot ID P04406

Calculated MW

location