Anti-MLH1 antibody

$245.00

  • Model: PAab05213
  • 50 Units in Stock
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Anti-MLH1 antibody

Product Name Anti-MLH1 antibody

Catalog No PAab05213

Packing 100ug

Form liquid

Alternative Name COCA2

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS- heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Immunogen mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)

Specificity Human,Mouse ,Rat

Tested Application ELISA,WB,IHC,IP

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human ovary tumor usingMLH1 antibody at dilution of 1:50

WB use

HEK-293 cells were subjected to SDS PAGE followed by western blot with PAab05213(MLH1 antibody) at dilution of 1:500

Protein Information

Gene ID4292

Uniprot ID P40692

Calculated MW

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