Anti-PADI4 antibody

Product Name Anti-PADI4 antibody

Catalog No PAab06105

Packing 100ug

Form liquid

Alternative Name PAD4 antibody, PADI5 antibody, PDI5

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg- 54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.

Immunogen peptidyl arginine deiminase, type IV

Specificity Human,Mouse ,Rat

Tested Application ELISA,WB,IHC

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using PADI4 Antibody at dilution of 1:200

WB use

A549 cells were subjected to SDS PAGE followed by western blot with PAab06105( PADI4 Antibody) at dilution of 1:600

Protein Information

Gene ID23569

Uniprot ID Q9UM07

Calculated MW

location