Anti-PKM2-specific antibody

$275.00

  • Model: PAab06495
  • 50 Units in Stock
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Anti-PKM2-specific antibody

 

Product Name Anti-PKM2-specific antibody

Catalog No PAab06495

Packing 100ug

Form liquid

Alternative Name CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PK-M2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

PKM2, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM2 plays a general role in caspase independent cell death of tumor cells. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells.The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2. PKM2, Pyruvate kinase isozymes M1/M2, has 2 isoforms .The immunogen of this antibody is M2 isoform, also called PKM2. This antibody is specific to PKM2 isoform.

Immunogen PKM2 pyruvate kinase, muscle

Specificity Human, Mouse

Tested Application ELISA,WB,IHC,IF,IP

Recommended dilution  WB: 1:1000-1:4000; IP: 1:200-1:1000; IHC: 1:100-1:400; IF: 1:10-1:100

IHC use 

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using PK-M2-specific Antibody at dilution of 1:200

WB use

MCF7 cells were subjected to SDS PAGE followed by western blot with PAab06495(PK-M2-specific antibody) at dilution of 1:1500

Protein Information

Gene ID5315

Uniprot ID  P14618

Calculated MW 58 kDa

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