Anti-UBE2B antibody
Product Name Anti-UBE2B antibody
Catalog No PAab09164
Packing 100ug
Form liquid
Alternative Name E2 17kDa antibody, HHR6B antibody, HR6B antibody, RAD6 homolog B antibody, RAD6B antibody, UBC2 antibody, UBE2B antibody, Ubiquitin carrier protein B antibody, Ubiquitin protein ligase B
Purification Immunogen affinity purified
Purity 95% as determined by SDS-PAGE
Host Rabbit
Isotype IgG
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys- 164'. May be involved in neurite outgrowth.
Immunogen ubiquitin-conjugating enzyme E2B (RAD6 homolog)
Specificity Human,Mouse ,Rat
Tested Application ELISA,IHC,WB
Recommended dilution WB : 1:200-1:2000;IHC:1:10-1:100
IHC use
Immunohistochemistry of paraffin-embedded human ovary tumor usingUBE2B antibody at dilution of 1:10
WB use
L02 cells were subjected to SDS PAGE followed by western blot with PAab09164( UBE2B Antibody) at dilution of 1:600
Protein Information
Gene ID7320
Uniprot ID P63146
Calculated MW 17kDa
location