Anti-UPF3B antibody

Product Name Anti-UPF3B antibody

Catalog No PAab09272

Packing 100ug

Form liquid

Alternative Name HUPF3B antibody, hUpf3p X antibody, MRXS14 antibody, RENT3B antibody, UPF3B antibody, UPF3X

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation; the function is independent of association with UPF2 and components of the EJC core.

Immunogen UPF3 regulator of nonsense transcripts homolog B (yeast)

Specificity Human,

Tested Application ELISA,WB,IHC

Recommended dilution WB: 1:500-1:5000; IHC：1:20-1:200

Validation:

Immunohistochemistry of paraffin-embedded human testis slide using UPF3B Antibody at dilution of 1:50.

Jurkat cells were subjected to SDS PAGE followed by western blot with UPF3B antibody at dilution of 1:2000.