Anti-USP13 antibody

Product Name Anti-USP13 antibody

Catalog No PAab09308

Packing 100ug

Form liquid

Alternative Name Deubiquitinating enzyme 13 antibody, Isopeptidase T 3 antibody, IsoT 3 antibody, ISOT3 antibody, Ubiquitin thiolesterase 13 antibody, USP13

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.

Immunogen ubiquitin specific peptidase 13 (isopeptidase T-3)

Specificity Human,Mouse ,Rat

Tested Application ELISA,WB,IHC,IP

Recommended dilution WB : 1:500-1:5000;IHC:1:20-1:200;IP:1:200-1:2000

IHC use

Immunohistochemistry of paraffin-embedded human testis usingUSP13 antibody at dilution of 1:50

WB use

HEK-293 cells were subjected to SDS PAGE followed by western blot with PAab09308(USP13 antibody) at dilution of 1:400

Protein Information

Gene ID8975

Uniprot ID Q92995

Calculated MW 97kDa

location