Anti-WDR61 antibody

$275.00

  • Model: PAab09500
  • 50 Units in Stock
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Anti-WDR61 antibody

Product Name Anti-WDR61 antibody

Catalog No PAab09500

Packing 100ug

Form liquid

Alternative Name REC14 antibody, SKI8 antibody, WD repeat domain 61 antibody, WDR61

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.

Immunogen WD repeat domain 61

Specificity Human,Mouse ,Rat

Tested Application ELISA,WB,IHC,IF

Recommended dilution WB : 1:500-1:5000;IHC:1:20-1:200;IP:1:500-1:5000;IF:1:10-1:100

IHC use

Immunohistochemistry of paraffin-embedded human breast cancer slide usingWDR61 Antibody at dilution of 1:50

WB use

mouse liver tissue were subjected to SDS PAGE followed by western blot with PAab09500(WDR61 antibody) at dilution of 1:1000

Protein Information

Gene ID80349

Uniprot ID Q9GZS3

Calculated MW 33kDa

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