Anti-YAP1 antibody

$275.00

  • Model: PAab09559
  • 50 Units in Stock
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Anti-YAP1 antibody

Product Name Anti-YAP1 antibody

Catalog No PAab09559

Packing 100ug

Form liquid

Alternative Name 65 kDa Yes associated protein antibody, YAP antibody, YAP1 antibody, YAP2 antibody, YAP65 antibody, YKI antibody, Yorkie homolog

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750). Isoform 2: Isoform 2 and isoform 3 can activate the C- terminal fragment (CTF) of ERBB4 (isoform 3).

Immunogen Yes-associated protein 1, 65kDa

Specificity Human, Mouse ,Zebrafish

Tested Application ELISA,IHC,FC,IF,IP,WB

Recommended dilution WB:1:500-1:5000;IHC:1:50-1:200;IF:1:20-1:200;IP:1:200-1:2000

IHC use

Immunohistochemistry of paraffin-embedded human ovary tumor usingYAP1 antibody at dilution of 1:100

WB use

HeLa cells were subjected to SDS PAGE followed by western blot with PAab09559( YAP1 Antibody) at dilution of 1:1000

Protein Information

Gene ID10413

Uniprot ID P46937

Calculated MW 54kDa,Observed 69kDa

location

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