Polyclonal Antibody- Human ELN


  • Model: PA-RA-90003
  • 20 Units in Stock
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Polyclonal Antibody- Human ELN 
Size: 0.2ml (200ug/ml)
Immunogen: Synthetic peptide-KLH
Ig type: Human IgG
Elastic fibers, which are comprised primarily of elastin, endow loose connective tissue with a resilience that compliments the tensile strength of collagenous fibers. Elastin is the main component of the extracellular matrix of arteries, and it performs a regulatory function during arterial development by controlling proliferation of smooth muscle and stabilizing arterial structure. Elastin is composed largely of glycine, proline, and other hydrophobic residues, and it contains multiple lysine-derived crosslinks, such as desmosines, which link individual polypeptide chains into a rubber-like network. During aging, the elasticity of connective tissue becomes reduced because of the cross-linking of collagenous fibers with elastin. 
Anti-ELN antibody is a rabbit polyclonal antibody raised against Human ELN . This antibody has been selected for its ability to recognize Human ELN  in immunohistochemiscal staining, non cross-reactive with other members of the family. 
APPLICATION   Immunocytochemistry in formalin fixed cells:  1:100-500
                   Immunohistochemistry in formalin fixed frozen section:  1:100-500
Immunohistochemistry  in paraffin section:  1:50-200
Optimal working dilutions must be determined by end user.                                                                                                                                                             
CONTENTS     0.1M PBS, 30%glycerol.
STORAGE      Store at 4° C for frequent use. Reconstituted antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
1.    Characterization of peptides resulting from digestion of human skin elastin with elastase.  Getie M., Schmelzer C.E.H., Neubert R.H.H. Proteins 61:649-657(2005)
2.    Mass spectrometric characterization of human skin elastin peptides produced by proteolytic digestion with pepsin and thermitase.  Schmelzer C.E.H., Getie M., Neubert R.H.H. J. Chromatogr. A 1083:120-126(2005)

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