Polyclonal Antibody- Human N-terminal propeptide COL1A1 
FOR RESEARCH USE ONLY. 
PROUDUCT INFORMATION
 
Size: 0.1ml (200ug/ml)
Storage: -20° C
Immunogen: Synthetic peptide-KLH
Ig type: rabbit IgG
 
BACKGROUND  
Collagen is a group of naturally occurring proteins. In nature, it is found exclusively in animals. It is the main protein of connective tissue. It is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. In muscle tissue it serves as a major component of endomysium. Collagen constitutes 1% to 2% of muscle tissue, and accounts for 6% of the weight of strong, tendinous muscles. Gelatin, which is used in food and industry, is derived from collagen. Type-I collagen is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone.
SPEFICITY    
Anti- N-terminal propeptide COL1A1 antibody is a rabbit polyclonal antibody raised against N-terminal propeptide COL1A1. This antibody has been selected for its ability to recognize N-terminal propeptide COL1A1 in immunohistochemiscal staining, non cross-reactive with other members of the family. 
APPLICATION   Immunocytochemistry in formalin fixed cells:  1:100-500
                   Immunohistochemistry in formalin fixed frozen section:  1:100-500
Immunohistochemistry  in paraffin section:  1:50-200
Optimal working dilutions must be determined by end user.                                                                                                                                                             
CONTENTS     0.1M PBS, 30%glycerol.
STORAGE      Store at 4° C for frequent use. Reconstituted antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
REFERENCE     
1.    Rossert J, Terraz C, Dupont S (2001). "Regulation of type I collagen genes expression.". Nephrol. Dial. Transplant. 15 Suppl 6: 66–8.
2.    Karsenty G, Park RW. "Regulation of type I collagen genes expression". Int Rev Immunol 12 (2-4): 177–85.