Polyclonal antibody-Leptin antibody

$400.00

  • Model: PA-RA-07883
  • 20 Units in Stock
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Leptin antibody

 

Product Information

Catalog No.:                    PA-RA-07883

Size:                                 100μg

Alternative name:LEP Antibody,Leptin Antibody,OB Antibody,Obesity factor Antibody,

Form:                               liquid

Purification:                     Immunogen affinity purified

Purity:                              ≥95% as determined by SDS-PAGE

Host:                                Rabbit

IsoType:                           IgG

Storage:                           PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Background

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. Leptin is the most critical hormone in the homeostatic regulation of energy balance among those so far discovered. Leptin primarily acts on the neurons of the mediobasal part of hypothalamus to regulate food intake, thermogenesis, and the blood glucose level. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.

 

Immunogen information

Immunogen:                    leptin

Synonyms:                      FLJ94114, LEP, leptin, OB, Obese protein, Obesity factor, OBS

Calculated MW:              16 kDa

Uniprot ID :                    P41159

 

Application

Specificity:                      Human, Mouse; other species are not tested. Please decide the specificity by homology

Tested Application:         ELISA, WB, IHC

Recommended dilution:  WB : 1:200-1:2000
IHC : 1:20-1:200
 

Image:

 

 

 

 

 

Immunohistochemistry of paraffin-embedded human testis tissue slide using PA-RA-07883 ( LEP Antibody) at dilution of 1:50

 

 

 

 

human adipose tissue were subjected to SDS PAGE followed by western blot with  PA-RA-07883 (LEP antibody) at dilution of 1:500

 

 

 

 

 

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using PA-RA-07883 (Leptin antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

 

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