Porcine 1,25-dihydroxy vitamin D3 ELISA Kit

Starting at: $796.00

  • Model: ELA-E0915p
  • 20 Units in Stock
Ask a question


Please Choose:


Add to Cart:

Porcine 1, 25- dihydroxy vitamin D3 ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
1,25-dihydroxyvitamin D3,Calcitriol,1,25-dihydroxycholecalciferol

Search name
Porcine 1,25-dihydroxyvitamin D3 ELISA Kit,Porcine Calcitriol ELISA Kit, Calcitriol ELISA Kit

Intended use
Porcine 1,25-dihydroxyvitamin D3 ELISA Kit allows for the in vitro quantitative determination of general calcitriol,Calcitriol concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
Porcine 1,25-dihydroxyvitamin D3 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Calcitriol, During the reaction, Calcitriol in the sample or standard competes with a fixed amount of
biotin-labeled Calcitriol for sites on a pre-coated Monoclonal antibody specific to Calcitriol. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Calcitriol in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range15.6-1000pg/ml

Porcine 1,25-dihydroxyvitamin D3 ELISA Kit PDF manual download

Order it by email:sales@lifescience-market.com

No customer comments for the moment.

Add A Comment

Related Products

Cart  

No products

Total $0.00

Prices don't include postage.

Cart