Porcine Biglycan, BGN ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
BGN,Biglycan,biglycan proteoglycan; DSPG1; PG-S1; PGI; SLRR1A; bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; small leucine-rich protein 1A
Search name
Porcine BGN ELISA KIT,Porcine Biglycan ELISA KIT ,Porcine biglycan proteoglycan ELISA KIT ,Porcine DSPG1 ELISA KIT ,Porcine PG-S1 ELISA KIT ,Porcine PGI ELISA KIT ,Porcine SLRR1A ELISA KIT ,Porcine bone/cartilage proteoglycan I ELISA KIT ,Porcine bone/cartilage proteoglycan-I ELISA KIT ,Porcine dermatan sulphate proteoglycan I ELISA KIT ,Porcine small leucine-rich protein 1A ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of porcine biglycan,Biglycan concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biglycan, During the reaction, Biglycan in the sample or standard competes with a fixed amount of biotin-labeled Biglycan for sites on a pre-coated Monoclonal antibody specific to Biglycan. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Biglycan in the samples is then determined by comparing the O.D. of the samples to the standard curve.